高效液相色谱法测定氟尿嘧啶在人体内的血药浓度  

Detennination of Fluorouracil in Human Blood Serum by HPLC

作　　者：黄雪梅[1] 卢晓宁[1] 路玫[1] 于灿华[2] 黄琳 

机构地区：[1]广西壮族自治区人民医院,南宁530021 [2]桂林医学院

出　　处：《中国药师》2002年第6期345-346,共2页China Pharmacist

摘　　要：目的:建立血清中氟尿嘧啶浓度的HPLC测定方法。方法:血清经处理后,采用Nova-Pak C_(18)柱,流动相为甲醇-0.3％乙酸溶度(2:98),检测波长266 nm。结果:氟尿嘧啶浓度在0.226～36.16μg·ml^(-1)范围内与峰面积呈良好的线性关系,血清中药物最低检测浓度为0.028 25 μg·ml^(-1);高中低3种浓度的日内 RSD(n=5)分别为2.2％,2.4％和3.5％,方法回收率为99.8％,107.5％和91.7％。结论:本方法灵敏度高,操作简便易行,结果准确可靠,能满足氟尿嘧啶血药浓度的测定。Objective: To establish an HPLC method for the determination of fluorouracil in human blood serum. Method:Nova-Pak C18 column was used,methanol-0. 3% acetic(2:98)was served as a mobile phase, a detecting wavelength was at 266 nm. Results: A good linear relationship was obtained between the peak area and the concentration of fluorouracil from 0. 226 to 36.16ug. ml-1, and the lowest detecting concentration was 0.0282 5ug.ml-1 .The RSD of intra-day variations were 2.2% ,2.4% , 3.5% and the recoveries were 99.8% , 107.5% , 91.7% in the high, middle, and low concentrations, respectively. Conclusion:A simple, sensitive and selective method for the determination of fluorouracil serum concentration in human was established.

关 键 词：高效液相色谱法 测定 血药浓度 氟尿嘧啶 抗肿瘤药物 

分 类 号：R979.1[医药卫生—药品] R927.1[医药卫生—药学]