慢性移植物抗宿主病时的T淋巴细胞受体Vβ基因表达  

Expression of T-cell receptor Vβgene in the patients with graft vs host disease

作　　者：杜欣[1] 李扬秋[2] 李树浓[3] 翁建宇[1] 陆泽生[1] 苏建华[1] 林伟[1] 黄梓伦[1] 

机构地区：[1]广东省人民医院血液科,广州510080 [2]暨南大学血液病研究室 [3]中山大学医学院病理生理教研室

出　　处：《中华器官移植杂志》2002年第3期176-178,共3页Chinese Journal of Organ Transplantation

基　　金：广东省 1998年重点攻关基金项目资助 (A199817)

摘　　要：目的　探讨T淋巴细胞受体 (TCR)Vβ基因在慢性移植物抗宿主病 (cGVHD)时的表达。方法　应用逆转录聚合酶链反应扩增 9例cGVHD患者的外周血单个核细胞的 2 4个TCRVβ亚家族的CDR3,了解患者TCRVβ的表达情况 ,并对 3例GVHD患者使用霉酚酸酯 (MMF)前后TCRVβ基因表达进行分析。 结果　 9例患者中 ,7例表达TCRVβ3,6例表达Vβ2、Vβ8和Vβ2 3,其它分散在不同的Vβ亚家族中 ;3例使用MMF者 ,在GVHD发生前 ,有TCRVβ3及其它亚家族基因表达 ,MMF治疗后 ,GVHD得以缓解 ,Vβ3不表达 ,当GVHD再次发生时 ,基因复又表达。 结论　cGVHD的发生可能与TCRVβ3、Vβ2、Vβ8及Vβ2 3(尤其是Vβ3)基因的表达有关。Objective To investigate the expression of T cell receptor Vβ (TCR Vβ) gene in the patients with chronic graft vs host disease (cGVHD).Methods The CDR3 of TCR Vβ 24 subfamily genes from peripheral blood mononuclear cells were amplified in 3 patients with GVHD following allogeneic hematopoietic stem cell transplantation using RT PCR for detection of the expression of TCR Vβ. The expression of TCR Vβ gene was analyzed in 3 cases of cGVHD before and after use of MMF.Results Among the 9 cases, 7 cases expressed TCR Vβ3, 6 expressed Vβ2, Vβ8 and Vβ23, and others distributed in different Vβ subfamily. For 3 patients given MMF, TCR Vβ3 and other subfamilies were expressed before GVHD onset; After treatment with MMF, GVHD got remission and Vβ was not expressed. The re occurrence of GVHD could result in the expression of Vβ again.Conclusion The occurrent of cGVHD might be related with the expression of TCR Vβ3, Vβ2, Vβ8 and Vβ23, especially Vβ3.

关 键 词：慢性移植物抗宿主病 T细胞受体β 基因表达 

分 类 号：R617[医药卫生—外科学]