TNF-α对严重烫伤小鼠骨骼肌摄取葡萄糖作用的研究  

Study on Effect of TNF-α on Glucose Uptake of Muscle in Scalding Mice

作　　者：王占科[1] 胡新勇[1] 许霖水[2] 柴长春[1] 詹志荣[1] 王白岚[1] 

机构地区：[1]解放军第94医院,江西南昌330002 [2]第三军医大学生化教研室,重庆400037

出　　处：《现代诊断与治疗》2002年第3期140-142,共3页Modern Diagnosis and Treatment

摘　　要：目的　证实TNF α导致创伤胰岛素抵抗的机制。方法　以 40只用 3 0 %TBSAⅢ度严重烫伤小鼠为模型 ,分烫伤对照组和TNF α单克隆抗体注射组 ,观察骨骼肌用和不用胰岛素刺激的葡萄糖摄取水平的改变。结果　烫伤对照组基础葡萄糖摄取明显升高 ,但胰岛素依赖的葡萄糖摄取明显降低 ,TNF α单克隆抗体注射治疗组基础葡萄糖摄取能力明显下降 ,但胰岛素刺激的葡萄糖摄取明显升高。结论　TNF α能促进骨骼肌基础葡萄糖摄取 ,但抑制胰岛素刺激的葡萄摄取。TNFObjective To study the mechanism of insulin resistance in scalding mice.Method 40 mice with 30% TBSA Ⅲ° scalding were randomly divided into scalding control group(n=20) and scalding treatment group(which were treated with TNF α McAb,n=20).The changes of the ability of glucose uptake of the muscle with and without insulin treatment were observed between above two groups.Results In scalding control group,the ability of glucose uptake of muscle of scalding mice increaed obviously,but that of the scalding mice with insulin treatment decreased markedly.In scalding treatment group,the ability of glucose uptake of muscle without insulin treatment reduced oubiously,and that in insulin treatment incresed markedly.Conclusions TNF α could enhance the ability of glucose uptake of muscle,but inhibites that in insulin treatment.TNF α plays an important role in the mechanism of insulin resistance in trauma.

关 键 词：TNF-Α 严重烫伤 小鼠 骨骼肌 胰岛素抵抗 葡萄糖 骨 

分 类 号：R644[医药卫生—外科学]