HPLC-蒸发光散射检测法测定注射用头孢他啶中碳酸钠的含量  

HPLC - ELSD Determination of Sodium Carbonate in Ceftazidime for Injection

作　　者：刘浩[1] 仇仕林[1] 王红武[2] 

机构地区：[1]上海市药品检验所,上海200233 [2]华东理工大学,上海200237

出　　处：《药物分析杂志》2002年第3期225-228,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立高效液相-蒸发光散射检测法测定注射用头孢他啶中碳酸钠的含量。方法:采用Hypersil SCX柱,以0.02mol·L^(-1)醋酸铵溶液(用冰醋酸调节pH至4.8)-乙腈(70:30)为流动相,流速为1.5mL·min^(-1),蒸发光散射检测,检测器温度:40℃,雾化气体(空气)压力:0.35 MPa。结果:Na+在50～501μg·mL^(-1)范围内呈良好的线性关系,r=0.999 4;平均回收率为100.1％,RSD=0.3％,n=6 ; 日内精密度(RSD=1.0％,n=6)良好。结论:本方法简便、快速,结果准确、可靠,重现性好。Objective: To establish HPLC - evaporative light scattering detection (ELSD) method for the deter-mination of sodium carbonate in ceftazidime for injection. Methods: The column; Hypersil SCX column (250 mm ×4. 0 mm, 5 μm) ; The mobile phase; 0. 02 mol ·L-1 ammonium acetate solution (adjusted to pH 6. 0 with glacial acetic acid) -acetonitrile (80:20); ELSD, the detector temperature; 40℃, the pressure of nebulizing gas (air) ; 0. 35 MPa. Results: The Standard curve was rectilinear within the range of 50 -501 μg · mL-1, r = 0.9994; The average recovery was 100. 1% (RSD=0. 3%, n =6); The precision in a day (RSD=1.0%, re = 6) was good. Conclusion: The method is simple and rapid, the results is accurate and good reproducible.

关 键 词：注射用 头孢他啶 碳酸钠 HPLC-蒸发光散射检测法 含量测定 

分 类 号：R978.11[医药卫生—药品] R927.2[医药卫生—药学]