大鼠口服茯苓素后血浆中的去氢土莫酸药代动力学研究  

Pharmacokinetic Study of Dehydrotumulosic Acid in Rat Plasma after Oral Administration of Poriatin

作　　者：宋宗华[1] 毕开顺[1] 王瑾[2] 冯东 

机构地区：[1]沈阳药科大学药学院,110016 [2]中国药品生物制品检定所,100050 [3]北京中顺制药厂,101300

出　　处：《药物分析杂志》2002年第3期228-231,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立测定大鼠血浆中去氢土奠酸浓度的高效液相色谱方法,并以去氢土莫酸为指标,研究大鼠灌胃给予茯苓素混合提取物后的药代动力学行为。方法:采用HPLC方法测定大鼠灌胃给予茯苓素混合提取物后,血浆中去氢土莫酸的浓度。色谱柱为ODS枉(200mm×4.6 mm,5μm),流动相为甲醇乙腈-2％冰醋酸水溶液(13:12:10),流速为1.0mL·min^(-1),检测波长为242nm,进样量20μL,室温下操作,内标物为丙酸睾丸素。结果:在0.20～20.0μg·mL^(-1)范围内具有良好线性关系(r=0.9981),方法回收率为85.2％～93.6％,日内、日间RSD均小于6.0％,达峰时间约为2h,峰浓度为(10.4±1.4)μg·mL^(-1),药时曲线下面积为32.6μg·mL^(-1)·h^(-1)。结论:此方法稳定、可靠,适用于茯苓素的药代动力学研究。Objective: RP - HPLC method was established to detect the plasma concentration of dehydrotumulosic acid in rat to study its pharmacokinetics after oral administration of poriatin, a mixed extraction of Poria. Meth-ods: The dehydrotumulosic acid concentration was determined by RP - HPLC with a mixture of methanol - acetoni-trile-2% aqueous solution of glacial acetic acid (13: 12: 10) as mobile phase, and Hypersil ODS column (200 mm×4. 6 mm, 5 μm) as stationary phase, detected at UV (λ =242 nm) , sampled with a 20 μL injection loop, all parts were operated at room temperature and testosterone propionate was used as the internai Standard. Results: The calibration curve was linear in the range of 0. 20 - 20. 0 μg · mL-1 ( r - 0. 998 1 ). The recovery was 85. 2% - 93. 6% . The RSD within - day and day - to - day were less than 6. 0% , tmax was 2 h and Cmax was ( 10. 4 ± 1.4) μg ·mL-1, AUC0-t and AUC0-00 were 29.5 and 32.6μg ·mL-1 · h-1 respectively. Conclusion: This method is stable and reliable, and it can be used to study the pharmacokinetics of poriatin.

关 键 词：茯苓素 血浆 去氢土莫酸 药代动力学 

分 类 号：R285[医药卫生—中药学] R969.1[医药卫生—中医学]