一株牙鲆皮肤溃烂症病原菌的鉴定  

Classification for One Pathogenic Vibrio anguillarumStrain Isolated From Skin-ulcer Flounder

作　　者：莫照兰[1] 茅云翔[2] 陈师勇[1] 张培军[1] 

机构地区：[1]中国科学院海洋研究所实验海洋生物学开放实验室,青岛266071 [2]青岛海洋大学海洋生命学院,青岛266003

出　　处：《微生物学报》2002年第3期263-269,共7页Acta Microbiologica Sinica

基　　金：国家重点基础研究专项资助项目 (G1 9990 1 2 0 0 3)~~

摘　　要：从山东荣成养鱼场发病牙鲆分离到一株病原菌M3 ,革兰氏阴性 ,杆状 ,能运动 ,菌落半透明 ,用BIOLOG细菌鉴定系统不能鉴定。通过 1 6SrDNA序列分析和同源性检索发现M3菌株与弧菌属的同源性较高 ,为 94%～ 98%。系统发育学分析表明菌株M3与鳗弧菌关系最近 ,相似性为 99 6 % ,其生化性状也和鳗弧菌的特征相似 ,故可把M3定为鳗弧菌 (Vibrioan guillarum)。A pathogenic bacterial strain M3 revealed gram negative rod shape, motile and translucent clone was studied. This bacterium was isolated from the skin-ulcer flounder in the fish farm of RongCheng City, Shandong Provice, and could not be identified by BIOLOG ID SYSTEM. Comparative 16S rDNA sequence analyses revealed that strain M3 was most related to the genus of Vibrio. The overall similarity value between strain M3 and Vibrio species were 94% to 98%. Phylogentic analysis showed that M3 strain exhibited the highest level of similarity to Vibrio anguillarum , and the biological features of strain M3 were very similar to that of Vibrio anguillarum. Based on the results obtained, strain M3 was identified as Vibrio anguillarum.

关 键 词：牙鲆 皮肤溃烂症 病原菌 鉴定 鳗弧菌 16S RDNA 系统发育 

分 类 号：S941.42[农业科学—水产养殖] S943.399[农业科学—水产科学]