透明颤菌血红蛋白基因在金色链霉菌中的克隆与表达  

Cloning and Expression of Vitreoscilia Hemoglobin in Streptomyces aureofaciens

作　　者：孟春[1] 叶勤[2] 石贤爱[1] 邱荔 宋思扬[3] 郭养浩[1] 

机构地区：[1]福州大学生物与食品科学系 [2]华东理工大学生物反应器国家重点实验室 [3]厦门大学生命科学学院

出　　处：《微生物学报》2002年第3期305-310,共6页Acta Microbiologica Sinica

基　　金：福建省科委资助项目 ( 99 H 4 6 )~~

摘　　要：分别用质粒pJJ6 99与pUC1 9(vhb) ,pIJ70 2与pBR3 2 2 (vhb) ,构建大肠杆菌 链霉菌穿梭质粒 ,将透明颤菌血红蛋白基因转入金色链霉菌。在低溶解氧浓度下 ,透明颤菌血红蛋白的表达 ,可提高金色链霉菌氧的利用效率 ,产物合成比原始菌株提高 40 %～ 6 0 %。在局部低氧的环境中 ,采用四环素抗性基因启动子带动血红蛋白基因表达 ,可有效发挥透明颤菌血红蛋白的氧传递效率 。Vitreoscilla hemoglobin Gene was cloned in Streptomyces aureofaciens through E.coli-Streptomyces shuttle plasmids constructed by both pIJ699-pUC19(vhb) and pIJ702-pBR322(vhb). Under low dissolved oxygen conditions, expression of hemoglobin enhanced CTC yield of engineering strain more about 40%～60% that that of control by improving oxygen transmission in cells. Hemoglobin expression by Promoter of tetracycline resistance gene was more effective for oxygen transmission than that by its native promoter regulated by dissolved oxygen concentrations in environments of locally low concentrations of dissolved oxygen.

关 键 词：透明颤菌 血红蛋白基因 金色链霉菌 克隆 表达 四环素 抗性基因启动子 天然启动子 

分 类 号：Q786[生物学—分子生物学]