介绍一种简便的红细胞无氧糖酵解率测定方法  

A SIMPLE AND CONVENIENT METHOD FOR ASSAYING HUMAN RBCs GLUCOLYSIS RATE WITHOUT OXYGEN

作　　者：刘秀珍[1] 马恩普[1] 韩颖[1] 南雪[1] 任素萍[1] 刘安[1] 靳鹏[1] 

机构地区：[1]军事医学科学院九所二室,北京100850

出　　处：《中国应用生理学杂志》2002年第2期190-192,共3页Chinese Journal of Applied Physiology

基　　金：全军医药卫生科研指令性课题资助项目 (960 5 0 0 6)

摘　　要：目的 :为了简化现有的华伯氏呼吸仪检测红细胞无氧糖酵解的方法 ,进行此项研究。方法 :实验用葡萄糖试剂盒 ,高氯酸 ,可见光分光光度计 ,氮气瓶和摇床。实验分 4步进行 :任氏液。Trls -HCI溶液等试液的配制 ,任氏血的制备 ,红细胞无氧糖酵解率的测定和红细胞无氧糖酵解率的计算。结果 :用SOD保养液。在 4℃条件下保存入红细胞 75d时糖酵解率为 86 .2 %± 5 .0 % ,明显优于GMA保养液 39.2 %± 8.9%。结论 :不用华伯氏仪的红细胞无氧糖酵解率的测定方法 ,操作简便 ,方法准确可靠 。Aim: To convenience of the methods for assaying red blood cell glucolysis without oxygen condition in the studies. Methods: Reagent kit of glucose,perchloric acid, visible light prismatic photometer, battle of nitrogen and rocking bed are used in the studies. The process includes 4 steps prepare Tris-HCI solution and so on, assay of red blood cell glucolysis without oxygen condition and account of glucolysis rate. Results: Human red blood cells stored at 4℃ for 75 d, in SOD solution, the glucolysis rate is 86.2%± 5.0 %, distinctly better than GMA solution ( 39.2% ±8.9%). Conclusion:The methods of assaying glucolysis without oxygen condition not use Habea's apparatus. The operation is convenient and simple and its determinations can be performed in ordinary laboratory and is is accurate.

关 键 词：红细胞 无氧糖酵解率 测定方法 血液保存 

分 类 号：R457.12[医药卫生—治疗学]