水分胁迫对2种生态型芦苇(Phragmites communis)的可溶性蛋白含量、SOD、POD、CAT活性的影响  

The effects of water stress on soluble protein content,the activity of SOD,POD and CAT of two ecotypes of reeds (Phragmites communis)

作　　者：王俊刚[1] 陈国仓[1] 张承烈[1] 

机构地区：[1]兰州大学生命科学学院,兰州730000

出　　处：《西北植物学报》2002年第3期561-565,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家重点基础研究专项经费资助项目 ( G1 9990 1 1 70 0 )

摘　　要：研究利用 2种抗旱性迥异的芦苇为材料 ,用 PEG6 0 0 0进行水分胁迫处理 ,结果表明 ,抗旱性强的沙丘芦苇 (沙芦 )的可溶性蛋白含量明显低于沼泽芦苇 (水芦 ) ,约为水芦的 1 /5。但是 ,在受到 2 0 % PEG胁迫时 ,沙芦的可溶性蛋白含量有所上升 ,水芦的则稍微下降 ,在 3 0 %PEG胁迫时 ,水芦的可溶性蛋白含量显著下降 ,而沙芦则先升后降。沙芦的 3种自由基清除酶 (SOD、POD、CAT)的活性显著高于水芦。受到水分胁迫后 ,2种芦苇的 SOD、POD、CAT活性或升或降。但是 ,无论在 2 0 %还是 3 0 % PEG胁迫条件下 ,相对水芦而言 ,沙芦都保持较高的自由基清除酶活性 ,从而保证其较强的自由基清除能力 ,减轻自由基对植物细胞生物大分子如 DNA、蛋白质、脂肪酸的伤害 ,维持细胞正常的生命活动 。Two ecotypes of reeds were used as experimental material and stressed under PEG6000.The results showed that the drought-resistant reed (dune reeds) contained less soluble proteins than that of the drought-sensitive reed (swamp reeds).However,the soluble protein content of dune reeds declined less than that of swamp reeds under water stress.Dune reeds exhibited higher activity of SOD,POD and CAT when compared with swamp reeds.When two ecotypes of reeds were stressed with PEG,the activity of SOD,POD and CAT increased while that of POD decreased continually.In general,there were higher activities of SOD,POD and CAT in dune reeds than in swamp reeds.Therefore,the results suggest that the drought resistance of plants is closely related to their activity of ROS scavengers.

关 键 词：水分胁迫 生态型 芦苇 可溶性蛋白含量 SOD POD CAT活性 抗旱性 植物 

分 类 号：Q945.78[生物学—植物学] Q948.112.3