苜蓿组织培养中球形胚发生时特异蛋白质和同工酶分析  

The analysis of special proteins and isozymes in globular embryogenesis of alfalfa in tissue culture

作　　者：刘天磊[1] 江晓雯[1] 王仑山[1] 

机构地区：[1]兰州大学生命科学学院,兰州730000

出　　处：《西北植物学报》2002年第3期625-629,共5页Acta Botanica Boreali-Occidentalia Sinica

摘　　要：试验在苜蓿组织培养中 ,对球形胚形成过程中特异蛋白质表达的模式、过氧化物酶及酯酶同工酶酶谱变化进行研究 ,结果表明 :苜蓿组织培养中从胚性愈伤组织到球形胚发育的进程中 ,顺序消失和出现了 1 1种中小分子量多肽 ;过氧化物酶同工酶酶谱发生了显著的变化 ;酯酶同工酶酶谱变化不大 。The expression patterns of soluble proteins,peroxidase and esterase isozymes were analysed on globular embryogenesis in tissue cultures of alfalfa,it was concluded that from embryogenic callus to globular embryo genesis 11 peptides emerged and disappeared orderly,the patterns of peroxidase isozyme changed dramatically,esterase isozymes changed slightly while the total enzyme activity was important to keeping the potential of somatic embryogenesis.

关 键 词：苜蓿 组织培养 球形胚发生 特异蛋白质 同工酶分析 

分 类 号：S542.4[农业科学—作物学] Q944.4[生物学—植物学]