籼稻明恢63成熟种子愈伤组织的诱导及转基因水稻的抗性检测  

Callus Induction and Regeneration from Mature Seeds of Indica Rice Minghui 63 and Anti-fungal Assay of Transgenic Rice Plants

作　　者：王莉江[1] 明小天[1] 安成才[1] 苑华毅[1] 陈章良[1] 

机构地区：[1]北京大学蛋白质和植物基因工程国家重点实验室,北京100871

出　　处：《生物工程学报》2002年第3期323-326,T001,共5页Chinese Journal of Biotechnology

基　　金：国家重点基础研究发展规划"973"项目 (No .G2 0 0 0 0 16 2 0 4);国家转基因植物研究与开发专项 (No.J0 0 A 0 0 5 )资助~~

摘　　要：利用不含附加营养成分的 2 ,4 D培养基 (2mg L)诱导明恢 6 3的成熟种子 ,9天预诱导后获得了大量的愈伤组织。利用基因枪辅助的土壤农杆菌转化法将天花粉蛋白 (Trichosanthin ,TCS)基因转入籼稻明恢 6 3的愈伤组织 ,并通过再生 (含有 3mg L 6 BA ,0 .5mg LABA和 1mg LNAA的N6培养基 )获得了转基因植物。Southernblot分析和Westernblot检测证明外源基因已经插入到T0代明恢 6 3的基因组中并获得了表达。初步研究结果表明 。A large number of callus from mature seeds of indica rice minghui 63 were obtained through pre-induction on medium with 2mg/L 2,4-D but without inorganic and organic components for 9 days. Trichosanthin gene was transferred into indica rice minghui 63 by using agrobacterium with the help of bombardment and the transgenic plants were obtained by inducing regeneration. Southern and Western blot analysis showed that the trichosanthin gene had been transferred into genome of minghui 63 and expressed in rice plants. The anti-fungal assay suggested that transgenic rice plants enhanced resistance to infection of Pyricularia oryzae.

关 键 词：籼稻 明恢63 成熟种子 愈伤组织 诱导 转基因水稻 抗性 检测 天花粉蛋白 抗真菌 

分 类 号：S511.21[农业科学—作物学]