重组人乙型肝炎病毒S基因表达的体内外评价  

Evaluation on expression of recombinant S gene of human hepatitis B virus in vitro and in vivo

作　　者：杨瑞仪[1] 曾庆平[1] 符林春[1] 陈征途[1] 

机构地区：[1]广州中医药大学热带医学研究所生物技术研究室,510405

出　　处：《中华传染病杂志》2002年第2期101-104,共4页Chinese Journal of Infectious Diseases

摘　　要：目的　构建能表达乙型肝炎病毒 (HBV)S基因的DNA疫苗 ,并在离体与活体条件下评价重组S基因的表达。方法　将克隆的S X基因片段插入真核细胞表达载体 ,获得重组质粒 ,在离体转录系统与转染细胞系中表达重组S基因 ,并在小鼠中评价HBsAg激发抗 HBs的效果。 结果　S基因的表达已通过Northern印迹杂交、Western印迹杂交和ELISA(抗原及抗体检测 )予以证实。结论　S基因已成功地实现体外重组与表达。Objective To construct a DNA vaccine capable of expressing S gene of hepatitis B virus(HBV) and evaluate the expression of recombinant S gene in vitro and in vivo. Methods A cloned S X gene fragment was inserted into a eukaryote expression vector to construct a recombinant plasmid. The S gene was transcribed in vitro and expressed in a transfected cell line, and the efficiency of HBsAg in eliciting anti HBs was evaluated in mice. Results The expression of S gene was confirmed by Northern blotting, Western blotting, and ELISA(for both antigen and antibody detection). Conclusions The recombination and expression of S gene is achieved successfully in vitro.

关 键 词：乙型肝炎病毒 S基因 疫苗DNA 基因表达 

分 类 号：R373[医药卫生—病原生物学]