糖尿病维生素D受体基因多态性与骨密度相关性研究  

Association of vitamin D receptor gene polymorphism with bone mineral density in the patients with diabetes mellitus

作　　者：董砚虎 翟木绪 李长贵 王海燕 柳林 曲宁 王元善 司元国 

机构地区：[1]青岛市内分泌糖尿病研究所暨医院,266033 [2]山东省淄博市第三医院内分泌科

出　　处：《中华内分泌代谢杂志》2002年第2期111-115,共5页Chinese Journal of Endocrinology and Metabolism

基　　金：山东省科委 2 0 0 0年课题资助项目 (0 0 3 13 0 119)

摘　　要：目的　探讨糖尿病 (DM )患者维生素D受体 (VDR)基因多态性与骨密度 (BMD)及骨转化的关系。方法　采用PCR RFLP技术 ,检测 6 8例 2型DM患者、5 4例 1型DM患者和 6 2例健康人的VDR基因型。并采用双能X线吸收法测BMD ,放免法测血清BGP、降钙素和 2 5 (OH)D3 ,免疫放射法测血清完整PTH及Ⅰ型胶原羧基末端前肽。结果　 (1)DM组VDR基因型分布与健康组差异无显著性 ;(2 )健康组AA基因型者腰椎和大转子BMD高于aa型 ,股骨颈和Ward′s三角BMD高于aa和Aa型 (P <0 .0 5 ) ,而 2型DM组Aa基因型者股骨颈和大转子BMD高于aa型 ,1型DM组Aa基因型者仅股骨颈BMD高于aa型 (P <0 .0 5 ) ;(3)VDR基因TaqⅠ位点多态性与BMD无明显相关性。结论　DM患者VDR基因ApaⅠ位点的多态性与BMD存在相关性。Objective To investigate the association of vitamin D receptor (VDR) gene polymorphism with bone mineral density (BMD) and bone turnover in the patients with diabetes mellitus (DM) of North China. Methods PCR RFLP was used to detect VDR genotypes in 68 type 2 diabetic patients, 54 type 1 diabetic patients and 62 normal controls. BMD was measured by dual energy X ray absorptiometry. The serum levels of osteocalcin, calcitonin and 25 hydroxyvitamin D were determined by radioimmunoassay. The serum levels of parathyroid hormone and collagen type Ⅰ C terminal propeptide were determined by immunoradioassay. Results There was no difference in the distrubution of VDR genotypes between the diabetic and control groups. In control group, subjects with 'AA' genotype had higher BMD at all four sites of bone than those of subjects with 'aa' genotype (P<0.05). Subjects with 'Aa' genotype had higher BMD at the greater trochanter and the femoral neck in type 2 diabetic group (P<0.05), subjects with 'Aa' genotype had higher BMD at the femural neck in type 1 diabetic group (P<0.05). No difference of BMD was found among the subjects with different genotypes of VDR TaqⅠ allele. Conclusion In the patient with DM, ApaⅠ polymorphism of VDR gene is associated with BMD.

关 键 词：骨化三醇受体 多态现象 骨密度 糖尿病 骨质疏松 

分 类 号：R587.1[医药卫生—内分泌]