培养小鼠卵巢表面上皮细胞合成细胞外间质的研究  

THE STUDY OF EXTRACELLULAR MATRIX SYNTHESIS OF CULTURED MOUSE OVARIAN SURFACE EPITHELIUM

作　　者：钱晓菁[1] 许增禄[1] 徐健[1] 徐园园[1] 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所,北京100005

出　　处：《解剖学报》2002年第1期74-77,共4页Acta Anatomica Sinica

基　　金：高等学校博士学科点专项科研基金资助项目(970 4)

摘　　要：目的　研究培养状态下卵巢表面上皮 (ovariansurfaceepithelium ,OSE)的特性。　方法　细胞培养 ,天狼星红 偏振光法 ,生物素 抗生物素蛋白间接免疫荧光方法 ,原位杂交。　结果　体外培养的OSE可表达Ⅳ型胶原和间质金属蛋白酶 2 (matrixmetalloproteinase 2 ,MMP 2 )的mRNA ,合成、分泌Ⅰ型、Ⅳ型胶原和层粘连蛋白 (laminin ,LN)。　结论　OSE在体外培养时 ,可合成多种细胞外间质 (extracellularmatrix ,ECM)成分和间质金属蛋白酶 (MMP 2 ) 。Objective To investigate the character of cultured mouse OSE. Methods Cell culture,picrosirius\|polarization method,biotin\|avidin DCS system indirect immunofluorescence technique, in situ hybridization with dig\|labeling type Ⅳ collagen and MMP\|2 cDNA probe were used. Results Cultured OSE expressed type Ⅳ collagen and MMP\|2 mRNA,produced type Ⅰ and Ⅳ collagen and LN.Conclusion\ During cultured in vitro, OSE prouduced some ECM componants and MMP\|2,we suggested that OSE might play an important role in ovulation,and repairment after ovulation.\;[

关 键 词：卵巢表面上皮 细胞外间质 间质金属蛋白酶 

分 类 号：R322.65[医药卫生—人体解剖和组织胚胎学] R329.24[医药卫生—基础医学]