孕酮拮抗物RU486对妊娠小鼠子宫巨噬细胞的影响及与妊娠相关性的研究  

INFLUENCE OF PROGESTERONE ANTAGONIST-RU486 ON THE MACROPHAGES IN THE UTERI OF PREGNANT MICE AND ITS RELEVANCE TO PREGNANCY

作　　者：田兴辉[1] 邓泽沛[1] 钟秀会[2] 周占祥[1] 

机构地区：[1]中国农业大学动物医学院解剖学教研室,北京100094 [2]河北农业大学,保定071001

出　　处：《解剖学报》2002年第1期97-101,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 (3 9770 5 43 ) ;北京市自然科学基金资助项目 (6982 0 14 )

摘　　要：目的　研究孕酮拮抗物RU4 86对妊娠小鼠子宫巨噬细胞的影响及与妊娠的相关性。　方法　取孕4、10d的小鼠 ,皮下注射RU4 86 (每只 15 0mg L) ,对照组以等量生理盐水代替。在注射后 12、2 4、36h密切观察妊娠结果 ,并用免疫组织化学方法检测子宫内的巨噬细胞。　结果　孕 4d ,注射RU4 86可以完全阻止胚泡的着床 ,且注射后 12～ 36h有大量的巨噬细胞涌入子宫内膜、肌层和肌间血管层 ,细胞数量极显著地高于对照组 (P <0 0 0 1)。孕 10d ,RU4 86处理可以导致大多数胚胎吸收 ,并在处理后 4～ 36h子宫巨噬细胞的数量和分布发生急剧的变化 ,尤其是 12～ 36h细胞数量极显著地高于对照组 (P <0 0 0 1)。　结论　RU4 86诱导小鼠妊娠早期的着床失败和妊娠中期的胚胎吸收与大量巨噬细胞侵入子宫有密切的关系 ,拮抗孕酮的免疫抑制功能可能是RU4Objective The experiment was conducted to explore the influence of progesterone antagonist\|RU486 on the macrophages in the uteri of pregnant mice and its relevance to pregnancy. Methods On day 4 and day10 of gestation, each mouse was subcutaneously injected with 150mg/L of RU486 (treatment group) or the same volume of saline (control group). The macrophages were immunohistochemically identified in the uterus 12, 24, 36?h after injection. Results Injection of RU486 on day 4 gestation had completely blocked the implantation of blastocysts. A large number of macrophages were found in the endometrium, myometrium and blood vessel layer 12～36?h after RU486 injection, which was significantly higher than that of the control groups ( P< 0 001). On day10 of gestation, the injection of RU486 resulted in absorption of most of the embryos. The distribution and density of macrophages changed dramatically from 4 to 36?h after injection. The positive cells at 12～36?h were obviously more than those of the control groups ( P<0\^001). Conclusion\ The blockage of implantation at the early stage of gestation and the absorption of embryos at the middle stage of gestation induced by RU486 may be related to the influx of large quantity of macrophages into uterus. It is one of mechanisms for RU486 to block pregnancy to antagonize the function of immunological suppression of progesterone.\;[

关 键 词：孕酮 RU486 子宫巨噬细胞 免疫组织化学 

分 类 号：R714.14[医药卫生—妇产科学]