内毒素、地塞米松对体外培养大鼠肺泡巨噬细胞中NF_(-k)B活化的影响  

Effects of LPS and dexamethasone on NF_(-k)B activation in cultured rat alveolar macrophages

作　　者：李文军[1] 宗城[2] 吉天鹏[1] 杨晓东[1] 张宏[1] 张斌[1] 李二红[1] 赵广贺[1] 

机构地区：[1]解放军第159医院全军烧伤中心,河南驻马店463000 [2]第三军医大学西南医院烧伤研究所,重庆400038

出　　处：《细胞与分子免疫学杂志》2002年第1期13-15,共3页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的 探讨内毒素（ＬＰＳ）和地塞米松（Ｄｅｘ）对体外培养的大鼠肺泡巨噬细胞（ＡＭ）中ＮＦ－ＫＢ活化的影响，以及ＡＭ中ＮＦ－ＫＢ活化在急性肺损伤中的可能作用。方法 通过建立大鼠ＡＭ体外培养技术，应用免疫组化染色法，观察ＬＰＳ和Ｄｅｘ寸 ＡＭ中 ＮＫ－ＫＢＰ～（６５），ＩＫＢ－α、ＴＮＦ－α和 ＩＣＡＭ－ｌ蛋白表达的动态变化。结果 ＬＰＳ能使培养的ＡＭ中 ＮＦ－ＫＢＰ～（６５），ＴＮＦ－α和ＩＣＡＭ－ｌ的表达增加，ＩＫＢ－α的表达降低；Ｄｅｘ的作用与 ＬＰＳ恰相反。结论ＬＰＳ促进ＡＭ中的ＮＦ－ＫＢ活化，可能是急性肺损伤肺内炎症损害发生的启动及促进因素；Ｄｅｘ抑制 ＮＦ－ＫＢ的活化，可能是其抗炎作用的重要机制之一。Aim To explore the effects of LPS and Dex on NF- KB activation of cultured alveolar macrophages ( AMs) and to study the role of AMs in the pathogenesis of acute lung injury. Methods AMs were harvested by bronchoalveolar lavage technique ,and cul- tured in vitros. The influences of LPS and Dex on expression of NF- KB^(65) ,IKB- α、TNF- α and ICAM- 1 proteins in the AMs were evalu- ated at the different time points ( 2,6, 12 and 24 hours after cul- ture) by immunohistochemical staining. Results LPS could in- crease the expression of NF- KB P^(65),TNF- α、and ICAM- 1 proteins and decrease 1KB- α in the AMs. The influences of Dex on the ex- pression of these proteins were contrary to those of LPS. Conclu sion LPS can promote NF- KB activation in AMs , which may contribute to the pathogenesis of acute lung injury. Dex can inhibit NF- KB activation in AMs ,which may be a mechanism of anti - in- flammation by Dex.

关 键 词：肺泡巨噬细胞 地塞米松 内毒素 NF-κB 细胞间粘附分子1 TNF-α IΚB-Α 

分 类 号：R392.11[医药卫生—免疫学]