中药狼疮方对狼疮样小鼠脾细胞体外分泌白细胞介素-6和白细胞介素-10的影响  

Effect of Lupus Recipe on IL-6 and IL-10 Secretion of Splenic Cells in Vitro in Lupoid Mice

作　　者：梁鸣[1] 李幼姬[1] 阳晓[1] 魏毅[2] 许韩师[1] 叶任高[1] 

机构地区：[1]中山医科大学附属第一医院肾内科卫生部肾脏病临床研究重点实验室,广州510080 [2]第一军医大学中医系

出　　处：《中国中西医结合杂志》2002年第5期372-375,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目 (No.39970 933) ;中山医科大学 2 1 1重点科研基金资助项目 (No.981 50 ) ;广东省自然科学基金资助项目(No .990 0 73) ;广东省中医管理局资助项目 (No .990 0 73)

摘　　要：目的 :探讨狼疮样小鼠脾细胞体外分泌白细胞介素 6 (IL 6 )和白细胞介素 10 (IL 10 )水平及其中药狼疮方对其影响。方法 :采用慢性移植物抗宿主病小鼠模型 ,经中药狼疮方、强的松及联合治疗后分别检测抗ds DNA抗体、刀豆蛋白A(ConA)诱导的脾细胞增殖反应和IL 6、IL 10水平。结果 :(1)脾细胞自身增殖反应性 ,模型组明显高于正常组 (P <0 0 5 ) ;各治疗组治疗后脾细胞自身增殖反应性与正常组比较差异不显著 (P >0 0 5 )。 (2 )模型组血清抗ds DNA抗体为 1 75± 0 2 5 ,与正常组 (1 2 0± 0 2 1)比较差异有显著性 (P <0 0 1)。各治疗组血清抗ds DNA抗体与正常组比较差异不显著 (P >0 0 5 )。 (3)模型组狼疮样小鼠脾细胞经ConA诱导下分泌Th2细胞因子IL 6和IL 10水平明显高于正常组及各治疗组 ,差异有显著性(P <0 0 5 )。结论 :狼疮方具有免疫抑制剂作用 ,可抑制T和B细胞活化 ,减少Th2细胞因子的形成和自身抗体产生 ,有效地治疗系统性红斑狼疮。Objective: To investigate the effect of Lupus Recipe (LR) on IL 6 and IL 10 secretion of splenic cells in vitro in lupoid model mice and on anti dsDNA antibody. Methods: Chronic graft versus host disease model was used in the experiment. The model mice were divided into four groups, the model group was un treated and the other three groups treated with LR, prednisone and combined treatment (prednisone+LR) respectively. The serum level of ds DNA antibody, the ConA induced splenic cell proliferation in mice′s splenic cell culture as well as the IL 6, IL 10 level in the supernatant of culture were determined after treatment and compared with those of normal controls. Results: (1)The splenic cell proliferative reaction in the model group splenic cells was obviously higher than that of the normal control (P<0 05); but that in the three treated groups was different from the control insignificantly (P>0 05); (2) The serum anti dsDNA in the model group was higher than that in the normal control, 1.75±0.25 vs 1.20±0.21 (P<0 01), while the difference in comparison of the treated groups with the normal control was insignificant, (P>0 05); (3) Splenic cell IL 6 and IL 10 secretion in the model group induced by ConA was higher than those in the treated groups and the controls significantly (P<0 05). Conclusion: LR reveals the effect of immunosuppressor, which could inhibit the activation of T and B cells, reduce the Th2 cytokine formation and auto antibody production so as to treat lupus erythematosus effectively.

关 键 词：狼疮方 白细胞介素-6 白细胞介素-10 系统性红斑狼疮 慢性移植物抗宿主病 细胞因子 发病机理 中医药疗法 实验研究 

分 类 号：R285[医药卫生—中药学]