多氯联苯对体外培养大鼠海马神经元毒性的研究  

Effection of polychlorinated biphenyls in cultured hippocampal neuronic cells of rats

作　　者：赵红斌[1] 史智勇[1] 王宇红 常德辉[1] 梁涛[1] 

机构地区：[1]兰州军区兰州总医院,甘肃兰州730050 [2]兰州市卫生防疫站

出　　处：《西北国防医学杂志》2002年第3期171-173,共3页Medical Journal of National Defending Forces in Northwest China

基　　金：甘肃省自然科学基金资助项目 (ZR -970 98)

摘　　要：目的 :研究多氯联苯 (PCB)对体外培养大鼠海马神经元细胞生长、存活的影响。方法 :体外法培养大鼠海马神经元细胞经不同剂量PCB共培养后 ,用MTT法观察海马神经元细胞生长、发育。结果 :高浓度的PCB明显抑制海马神经元细胞的存活率 ,10 -4 mol·L-1组同对照组相比相差极显著 (P <0 .0 1) ;10 -7mol·L-1、10 -6mol·L-1组同对照组相比相差显著 (P <0 .0 5 ) ,海马神经元细胞存活率明显降低。结论Objective:To evaluate the effect of Polychlorinated Biphenyls(PCB) in cultured hippocampal neuronic cells of rats.Methods:After rats' hippocampal cells were cultured with different PCB, MTT was used to observe the growth and development of hippocampal cells.Results:It was obvious that hippocampal neuronic cells was inhibited in the highest groups of PCB,10 -4 group was apparently lower than that of control group(P<0.01);10 -7 and 10 -6 groups were lower than that of control group(P<0.05);The live rate of cell was reduced.Conclusion:PCB had strong nerve toxicity to hippocampal neuronic cells.

关 键 词：神经性毒剂 多氯联苯 海马神经元细胞 大鼠 

分 类 号：R965[医药卫生—药理学]