一个新的水稻GST类似蛋白基因XIG的克隆与分析  

Cloning and Analysis of the Rice Gene Coding a New GST-like Protein

作　　者：张翼飞[1] 史晰[1] 王喜萍[1,2] 曹凯鸣[1,3] 

机构地区：[1]复旦大学生命科学学院 [2]上海200433 [3]上海

出　　处：《复旦学报（自然科学版）》2002年第1期27-30,35,共5页Journal of Fudan University：Natural Science

基　　金：上海市现代生物与新药产业发展基金 (994 3192 15 1);国家转基因植物研究与产业化基金 (J99 A 0 4 4 )

摘　　要：根据编码一种玉米GST类似蛋白的mRNAIn2 1序列设计引物 ,以水稻cDNA文库为模板 ,PCR扩增得到一条 2 70bp的DNA片段 .以此片段为探针分别筛选水稻根cDNA文库及水稻基因文库 ,获得一条全长cDNA及其相应的全长基因 ,并通过测序得到了两者的全序列 .该基因编码的蛋白具有GST的典型特征 。A pair of primers were designed according to the sequence of In 2 1 mRNA that was coding a GST like protein in Maize. A DNA fragment of 270 base pairs was amplified from a rice root cDNA library by the means of PCR. Using the PCR fragment as the probe for screening, a complete cDNA and its complete gene were obtained from that cDNA library and a rice gene library, respectively. Both of the complete cDNA and the complete gene were sequenced. The protein, coded by the newly reported rice gene, showed typical characteristics of GSTs.

关 键 词：水稻 谷胱甘肽-S-转移酶 蛋白质结构 

分 类 号：Q751[生物学—分子生物学] S511[农业科学—作物学]