水稻苹果酸脱氢酶基因的克隆及其在E.coli中的表达  

Cloning of the Rice Cytoplasmic Malic Dehydrogenase Gene ( Rc MDH ) and Its Expression in E.coli

作　　者：姜瑞华[1] 林长发[1] 申国安[1] 钱旻 王东[1] 杜喜玲[1] 黄骥[1] 杨金水[1] 

机构地区：[1]复旦大学生命科学学院,遗传学研究所上海20043

出　　处：《复旦学报（自然科学版）》2002年第1期67-69,共3页Journal of Fudan University：Natural Science

基　　金：教育部重点资助项目

摘　　要：以玉米苹果酸脱氢酶的基因为探针 ,从水稻幼苗cDNA文库筛选得到一条水稻新基因 .它与玉米ZHO2序列的一致性高达 87% ,被命名为RcMDH(ricecytoplasmicmalicdehydrogenasegene) .分析发现RcMDH具有完整的读码框 ,编码 332个氨基酸 ,预计分子质量为 36 .5ku .导入大肠杆菌中表达 。Using the maize MDH gene as probe, a novel rice full length cDNA was cloned through screening a rice seedling cDNA library. It showed 87% identity in nucleic acid sequence to ZHO 2(GenBank No.AF007581)of maize, and therefore was named as rice cytoplasmic malic dehydrogenase gene( RcMDH ). This gene encodes 322 amino acids, with putative molecular weight of 36.5 ku. The open reading frame of RcMDH was subcloned into reconstructed pGEX 4T 1 plasmid which expressed in E.coli with IPTG inducing. The MDH enzyme activity of its product was also certified.

关 键 词：水稻 细胞质 莱果酸 脱氢酶基因 克隆 表达 

分 类 号：Q78[生物学—分子生物学] S511[农业科学—作物学]