一条新的人类组氨酰tRNA合成酶类似物全长cDNA的克隆及表达谱分析  

Cloning and Expression of a Novel cDNA Coding for the Analogue of Human Histidyl-tRNA Synthetase

作　　者：孟宪芳[1] 陈金中[1] 杨泉胜 王曙[1] 周光云[2] 应康[1] 谢毅[1] 毛裕民[1] 

机构地区：[1]复旦大学生命科学学院遗传学研究所,上海200433 [2]同济医科大学生物研究室,武汉430030

出　　处：《复旦学报（自然科学版）》2002年第1期75-78,共4页Journal of Fudan University：Natural Science

基　　金：上海市现代生物与新药产业发展基金资助项目

摘　　要：从人胎脑文库中克隆到一条长为 12 19bp的cDNA ,它编码 2 3.4ku的肽链 .该肽链与葡萄球菌组氨酰tRNA合成酶N端同源 5 8% .利用humangenomicblast可将其定位于 2 0p11.Northern杂交显示胎脑中有一约1.5kb的单一条带 .基因芯片杂交分析表明 ,其在肺癌和前列腺癌组织中表达较高 .A cDNA coding for a putative analogue of human histidyl tRNA synthetase was isolated by screening human 18 week old embryo brain cDNA library. This novel cDNA with the length of 1 219 bp contained an open reading frame (ORF) of 209 amino acids, with deduced molecular mass of 23.4 ku. Northern blot analysis revealed it was a full length cDNA. Expression profile analysis indicated that the novel cDNA was highly expressed in lung cancer and prostate cancer. The gene was expressed and located in cytoplasm when its ORF was fused with pEGFP in COS7 cell.

关 键 词：组氨酰tRNA合成酶类似物 克隆 表达 

分 类 号：Q751[生物学—分子生物学] Q559