椴木尘的遗传毒性初探  

Study on hereditary toxicity of bass wood dust

作　　者：吴萍[1] 张杰[1] 苏忆兰[1] 韩春华[1] 王起恩[2] 卢景芬[3] 

机构地区：[1]首都医科大学环境与劳动卫生教研室,北京100054 [2]北京大学医学部劳动卫生与环境卫生学系 [3]天然药物及仿生药物国家重点实验室

出　　处：《中华劳动卫生职业病杂志》2002年第2期119-121,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的　探讨椴木尘的遗传毒性。方法　检测某火柴厂以接触椴木尘为主的工人的外周血淋巴细胞微核率。用椴木尘浸出液对小鼠进行了微核、单细胞电泳试验和肝组织活性氧自由基的检测及脂质过氧化水平 [丙二醛 (MDA)含量 ]、红细胞超氧化歧化酶活力 (SOD)的测定。结果　工龄为0～、5～、≥ 10年的接触椴木尘工人的外周血淋巴细胞微核阳性率分别为 5 0 .0 %、5 1.9%、5 0 .0 %,与对照组 (4 .5 %)相比 ,差异有显著性 (P <0 .0 1) ;椴木尘浸出液染毒小鼠骨髓嗜多染红细胞微核率也呈明显的剂量 -反应关系 (r=0 .78,P <0 .0 1)。染毒小鼠的SOD活力 [(10 .98± 5 .74)、(15 .70± 7.5 4)、(2 9.6 3± 14.97) μg/gHb]及MDA含量 [(4 .93± 0 .90 )、(4 .6 1± 1.0 6 )、(4 .33± 0 .6 9)mmol/g肝 ]与对照组[(35 .80± 12 .92 ) μg/gHb、(2 .5 1± 0 .34 )mmol/g肝 ]相比 ,差异有显著性 (P <0 .0 5 ) ,并有明显的剂量 -反应关系 (SOD活力 :r=- 0 .6 7,P <0 .0 1;MDA含量 :r =0 .6 5 ,P <0 .0 1)。染毒小鼠肝细胞的单细胞电泳结果显示 ,随着染毒剂量的升高 ,DNA链的断裂有增高的趋势 ;肝组织中的活性氧自由基的信号也有随染毒剂量的升高而增高的趋势。结论　椴木尘可能存在一定的遗传毒性。Objective To explore the genotoxicity of bass wood dust. Methods Micronucleus frequency in peripheral lymphocytes of workers exposed to bass wood dust in a match factory were examined,solution of bass wood dust emmersion was prepared and the effect on micronucleus frequency in poly-dyeing red blood cell of mice's sternum marrow was also detected.Single cell gel electrophoresis assay was used to detect DNA damage in liver cell,the level of oxygen free radical,lipid peroxidation(MDA) in the liver and the activity of superoxide dismutase(SOD) in red blood cells were also studied. Results The positive frequency of micronucleus in bass wood-exposed workers with different length of service(0～,5～,≥10 a) were 50.0%,51.9%,50.0% respectively,significantly higher(P<0.01) than that of the control group(4.5%).A dose-effect relationship could also be found in the mice's micronucleus frequency study(r=0.78,P<0.01).The activities of SOD[(10.98±5.74), (15.70±7.54),(29.63±14.97)μg/g Hb] were significantly lower than that of control group[(35.80±12.92) μg/g Hb],and the level of MDA[(4.93±0.90),(4.61±1.06),(4.33±0.69)mmol/g liver] were significantly higher than that of the control group[(2.51±0.34)mmol/g liver].Single cell gel electrophoresis study showed DNA strand breaks increased with the dose increase and the level of oxygen free radical also increased with the dose increase. Conclusion Bass wood dust may have certain degree of genotoxicity.

关 键 词：遗传毒性 椴木尘 微核率 DNA损伤 活性氧自由基 脂质过氧化 超氧化物歧化酶 

分 类 号：R99[医药卫生—毒理学]