哮喘患者痰液中炎症介质和细胞因子与气道重塑的关系研究  

Relationship between airway remodeling and inflammatory mediators and cytokines in sputum in patients with bronchial asthma

作　　者：崔曼丽[1] 陈升汶 邱晨 陶维华 吴福成 陶俞[3] 

机构地区：[1]深圳市红会医院,sicu518020 [2]深圳市人民医院呼吸科 [3]中山医科大学病理教研室

出　　处：《中华结核和呼吸杂志》2002年第6期344-346,共3页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：目的　以支气管粘膜网状基底膜厚度作为气道重塑的指标 ,探讨哮喘患者气道重塑与痰液中细胞因子和炎症介质的关系。方法　对 2 0例哮喘患者和 10名正常对照者经纤维支气管镜行(纤支镜 )支气管粘膜活检 ,测量支气管粘膜网状基底膜厚度 ,用荧光酶联免疫方法测定痰液中的嗜酸细胞阳离子蛋白 (ECP) ,酶联免疫法测定白细胞介素 5 (IL 5 )、肿瘤坏死因子 (TNF α)的水平 ;采用SPSS8 0统计软件作等级相关分析 ,探讨哮喘患者痰液中ECP、IL 5、TNF α水平与支气管粘膜厚度的关系。结果　缓解期哮喘患者支气管粘膜网状基底膜厚度为 (10 1± 2 6 ) μm ,与正常对照组 [(4 4± 1 2 )μm]比较 ,差异有显著性 (P <0 0 0 5 ) ;哮喘组痰液中ECP水平为 (14 4± 80 ) μg/L、IL 5为 (17± 4 ) μg/L、TNF α为 (14 6± 79) μg/L ,与正常对照组 [(81± 4 4 ) μg/L、(14± 4 ) μg/L、(5 3± 36 ) μg/L]比较 ,差异有显著性 (P <0 0 0 5 ) ;两组痰液中ECP、IL 5与支气管粘膜厚度呈明显正相关 (r =0 5 6 9、0 4 6 6 ,P均 <0 0 0 5 ) ;两组痰液中TNF α水平与粘膜厚度无明显相关 (r=0 2 5 4 ,P >0 0 5 )。结论　缓解期哮喘患者支气管粘膜网状基底膜厚度均存在不同程度增厚 ;而痰液ECP和IL 5水平与支气管粘膜厚度呈?Objective To compare the thickness of subepithelial reticular basement membrane(BM) in asthmatic patients and healthy subjects, and to compare the BM thickness to inflammatory mediators and cytokines Methods Fiberoptic bronchoscopy with bronchial biopsy was performed on 20 stable asthmatics and 10 normal subjects The subepithelial reticular BM thickness was measured Levels of eosinophil cationic protein (ECP), interleukin 5 ( IL 5 ), and tumor necrosis factor α( TNF α ) in sputum were measured by immunoassay The relationship between ECP, IL 5, TNF α and BM thickness was estimated by the spearman rank correlation Results The mean BM thickness was significantly higher in asthmatic patients (10 1±2 6)μm than in normal subjects (4 4±1 2)μm ( P <0 005) In asthmatic patients, the ECP (144±80)μg/L and the IL 5 (17±4) μg/L levels in sputum were positively correlated with BM thickness ( r =0 569, P <0 005 and r =0 466, P <0 005, respectively ) Sputum TNF α(53±36) μg/L showed no significant correlation with BM thickness ( r =0 254, P >0 05) Conclusions Airway remodeling is a distinctive and characteristic pathologic finding of asthma Airway remodeling is related to levels of sputum ECPand IL 5, but not that of TNF α

关 键 词：哮喘 痰液 炎症介质 细胞因子 气道重塑 关系研究 

分 类 号：R562.250.2[医药卫生—呼吸系统]