乙型肝炎病毒D基因型系统进化树分析  

Analysis of the phylogenetic tree of human hepatitis B virus genotype D complete nucleotide

作　　者：阎丽[1] 侯金林[1] 郭亚兵[1] 林裕龙[1] 骆抗先[1] 牛贞玉 

机构地区：[1]第一军医大学南方医院感染内科 [2]宁夏回族自治区银川市兰州军区解放军第五陆军医院

出　　处：《中华微生物学和免疫学杂志》2002年第3期269-272,共4页Chinese Journal of Microbiology and Immunology

基　　金：973计划 (G1 9990 541 0 5) ;国家自然科学基金资助项目(39630 2 80 )

摘　　要：目的　研究HBVD基因型不同毒株全基因进化关系。方法　用聚合酶链式反应(PCR)、克隆及核酸序列测定的方法 ,测定了 1例中国人慢性无症状携带者感染的乙型肝炎病毒D基因型全基因序列。此D基因型毒株全基因序列GenBankAccession为AF2 80 817,将GenBank中已发表的HBVD基因型 30株的全序列进行了系统进化树分析。结果　中国株HBVD基因型与源于瑞典 (Swe den)的 4株HBVD基因型全基因的进化距离最近 ;地中海地区及欧洲国家系HBVD基因型分布的优势地域 ,亚洲并非HBVD基因型分布的优势地域。结论　HBVD基因型病毒株传入来源。Objective To study the phylogenetic relationship of different strains of HBV D genotype complete nucleotide. Methods By HBV full length polymerase chain reaction(PCR) in combination with cloning and nucleotide sequencing assay. A Chinese strain of hepatitis B virus genotype D complete nucleotide clone from a chronic asymptomatic HBV carrier of infectious HBV strain of genotype D was sequenced, and GenBank accession of its complete nucleotide sequence is AF280817. Phylogenetic tree was obtained by analysis of complete genes of 30 strains of HBV genotype D sequences from NCBI by use of DNASIS and CLUSTALW. Results The evolutional distance of this Chinese HBV strain of genotype D is the most homogenous to four Swedish HBV strains of genotype D in 30 strains of HBV genotype D published by GenBank. Mediteranean area and Europe countries but not Asia are predominant geographical region of HBV genotype D. Conclusion The discrepancy of evolution of HBV strain of genotype D result from the origin of virus, the distinctness of migratory orientation of virus and the long choice of virus in the host of different heredity and immune idiosyncracy were discussed. [

关 键 词：乙型肝炎病毒 基因型 序列分析 进化树 

分 类 号：R373[医药卫生—病原生物学]