人gp130结合分子与TLE1分子通过同源的Q结构域相互作用  

Interaction between gp130 associated molecule and transducin like enhancer of split 1 through their homologous Q domain

作　　者：刘飞[1] 金伯泉[1] 刘崟[1] 李德敏[1] 朱勇[1] 欧阳为明[1] 谢鑫[1] 

机构地区：[1]第四军医大学免疫学教研室,西安710032

出　　处：《中华微生物学和免疫学杂志》2002年第3期303-307,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金资助项目 (39880 0 1 1 )

摘　　要：目的　确定人gp130结合分子 (GAM)与transducinlikeenhancerofsplit 1(TLE1)分子间是否存在相互作用及相互作用的结构基础 ,以深入研究这两种分子的功能。方法　扩增编码GAM与TLE1分子不同结构域的cDNA ,构建含有这些不同结构域的酵母双杂交载体 ,通过酵母双杂交系统分析和免疫共沉淀试验 ,研究这两种分子间的相互作用。结果　DNA序列测定证实成功构建了含GAM与TLE1分子不同结构域的酵母双杂交载体 ,酵母双杂交分析表明GAM与TLE1分子通过氨基端的Q结构域相互结合 ,免疫共沉淀试验证实了这一发现。Objective To determine whether gp130 associated molecule(GAM) interacts with Transducin Like Enhancer of split 1 (TLE1) molecules, and locate the domains involved in this interaction. Methods The cDNA fragments encoding various domains of GAM and TLE1 were amplified by PCR and cloned into the yeast duplicate hybrid vectors pGAD424 or pGBT9, yeast duplicate hybrid assay and immuno co precipitation assay were performed to verify the interaction between GAM and TLE1. Results The yeast duplicate hybrid vectors containing cDNAs of various domains of GAM and TLE1 were successfully constructed. Yeast duplicate hybrid assay and immuno co precipitation assay indicated that GAM and TLE1 interact by their homologous Q domain. Conclusion GAM and TLE1 interact by their homologous Q domain. [

关 键 词：gp130结合分子 信号传导 TLE1 蛋白质相互作用 免疫共沉淀试验 酵母双杂交系统分析 

分 类 号：R392[医药卫生—免疫学]