电针对MCAO大鼠脑内GAP-43和突触囊泡蛋白表达的影响  

Effect of Electroacupuncture on GAP-43 and Synaptophysin Expression of Ischemic Brain in the MCAO Rat

作　　者：黄显奋[1] 陈英辉[2] 

机构地区：[1]上海医科大学神经生物学教研室,上海200032 [2]上海第二医科大学附属新华医院神经内科,上海200093

出　　处：《中国针灸》2002年第6期413-416,共4页Chinese Acupuncture & Moxibustion

基　　金：本文为国家自然科学基金资助项目(No.39730510)

摘　　要：方法:将实验大鼠分为假手术组、缺血再灌组和缺血再灌+电针组,大脑中动脉线栓（MCAO）造成局灶性缺血90min,电针组在缺血后立即给予电针1h,以后每天1次。在缺血再灌1,7和14天分别处死,进行免疫组织化学染色。结果:GAP-43和突触囊泡蛋白主要在缺血灶周围的皮质表达。在再灌后7和14天后电针组GAP-43和突触囊泡蛋白免疫阳性细胞数高于缺血再灌组（P<0.05）。结论:电针能够提高GAP-43和突触囊泡蛋白在缺血灶周围皮质的表达,从而促进缺血后轴突的出芽和突触的形成,提高了缺血后神经元可塑性。Purpose To observe the effect of electroacupuncture (EA) on growth-associated protein (GAP-43) and Synaptophysin expression of ischemic brain in the rat by immunohistochemical method. Methods The experiment was divided into three groups: sham operation group, ischemia group and ischemia plus EA group. The middle cerebral artery (MCA) was occluded for 90 min to result in the local ischemia. EA was applied for one hour immediately after cerebral ischemia. Then EA was applied every day till the rats were decapitated after reperfusion for 1,7 and 14 days, respectively. Immunohistochemical method was used. Results GAP-43 and Synaptophysin immunoreactive cells expressed mainly in the surrounding cortex of infarction. In the EA group GAP-43 and Synaptophysin immunoreactive cells after reperfusion for 7 and 14 days were significantly higher than those in the ischemia group (P<0.05). Conclusion EA may increase expression of GAP-43 and Synaptophysin in the surrounding cortex of infarction, so EA can promote the neuronal sprouting and synaptogenesis after ischemia, and improve the neuronal plasticity.

关 键 词：电针 MCAO 脑内 GAP-43 突触囊泡蛋白 再灌注损伤 针灸疗法 脑缺血 

分 类 号：R246.6[医药卫生—针灸推拿学] R277.733.3[医药卫生—中医临床基础]