皮下注射血管内皮生长因子基因提高大鼠背部随意皮瓣活力的实验研究  

Subcutaneous injection of plasmid VEGF gene: a method of gene therapy to enhance the viability of random skin flap

作　　者：王法刚[1] 赵敏[1] 黄秉仁[2] 刘珍君[1] 熊斌[1] 成铤[1] 霍孟华[1] 

机构地区：[1]中国医学科学院中国协和医科大学整形外科医院 [2]中国医学科学院基础医学研究所

出　　处：《中华整形外科杂志》2002年第3期157-159,I001,共4页Chinese Journal of Plastic Surgery

摘　　要：目的　探索基因治疗在组织移植方面的应用。方法　构建并体外扩增表达质粒pcD NA3.1 Zeo(+) -VEGF16 5 ,以之直接皮下注射转染SD大鼠背部随意皮瓣模型。通过与对照组相比较 ,观察其对皮瓣活力的影响 ,并采集组织行免疫组化检查。结果　基因转染组在毛细血管周围及肌间隙可见VEGF的沉积 ,与对照组相比皮瓣的平均成活面积显著增加。结论　皮下注射的基因在组织中能够表达VEGF ,增加皮瓣活力 。Objective\ Gene therapy has been becoming one of the most attractive medical areas. But the using of gene therapy in plastic surgery is relatively scarce. Our purpose was to investigate the effect of naked plasmid encoding Vascular Endothelial Growth Factor on the viability of the random skin flap by directly injected subcutaneously. Methods\ 30 female Sprague Dawley rats randomly divided into three groups. A random dorsal skin flap of 3 cm×9 cm was elevated in each of the rats. And 1ml double distilled water solution was injected subcutaneously, which was only water in group 1 during the operation, 200 μg VEGF cDNA plasmid in group 2 during the operation, 200 μg pcDNA3.1/zeo(+)-VEGF in group 3,24 hours before the operation, respectively. 7 days after the operation, all the animals were sacrificed by overdose anesthetic. The survival tissue was measured with planimetry. Two samples were harvested from each group for pathological check and immunohistochemical test. Results\ Immunohistochemical staining demonstrated that there was human VEGF deposited around the capillary in the flaps treated with VEGF gene. The flaps treated with VEGF gene had a larger percentage of survival skin (group1=47%±5.4%, group2=65.4%±6.3%, group3=72 3%±8 5%, P <0 05). Conclusion\ VEGF gene directly injected into subcutaneous can express VEGF. It makes the gene therapy simple and practical and will be promising future in the tissue transplantation.\;

关 键 词：皮下注射 血管内皮生长因子 背部随意皮瓣 实验研究 基因治疗 

分 类 号：R622[医药卫生—整形外科]