p21抑制人血管平滑肌细胞增殖的研究  

Inhibition of proliferation of human vascular smooth muscle cells by overexpression of p21 gene

作　　者：张学利[1] 张群华[1] 余波[1] 王铁平[1] 张延龄[1] 蔡端[1] 李平[2] 朱运松[2] 

机构地区：[1]复旦大学附属华山医院普外科 [2]复旦大学附属华山医院分子遗传教研室

出　　处：《中华医学杂志》2002年第10期696-698,共3页National Medical Journal of China

基　　金：上海市青年科技启明星计划基金资助项目(0 0QB14 0 2 0 )

摘　　要：目的　探讨p2 1基因转染诱导体外培养的人血管平滑肌细胞 (HVSMC)凋亡进而抑制其增殖的可能作用机制。方法　通过腺病毒介导将外源性p2 1基因转入HVSMC ,利用印迹杂交分析、荧光染色分析等对p2 1基因的表达、靶细胞增殖抑制及凋亡、组织型转谷氨酰胺酶 (tTG)的表达进行分析。结果　外源性p2 1基因高水平的表达显著抑制了靶细胞的增殖 (P <0 .0 1) ,tTG表达的显著升高及荧光染色检测均提示靶细胞发生了凋亡。结论　p2 1基因的过度表达可诱导HVSMC凋亡并能抑制其增殖 ,这一过程可能与tTG的高表达有关。Objective To observe the apoptosis of human vascular smooth muscle cells (HVSMCs) transfected with p21 gene and investigate its potential mechanism. Methods An adenoviral expression vector with full length cDNA of p21 gene insert (Ad p21) was constructed and transfected into HVSMC. A mock vector, pAdeno X lac Z constructed with the same mothod, was transfected into HVSMCs as conrol. Western blotting and DS PAGE were used to detect the expression of p21 gene. Fluorescence staining was used to observe the changes in nuclei of apoptotic cells and calculate the cell survival rate. Expression of tissue transglutaminase(tTG) in HVSMC was examined by DIG marked probe. Results Fluorescence staning showed marked fragmentation of nucleus and pyknosis of chromatin in pAdeno X p21 transfected cells. The expression of tTG was at a higher level in pAdeno X p21 transfected cells than in normal cells and pAdeno X lac transfected HVSMCs. Conclusion Overexpression of p21 gene inhibits the proliferation of HVSMC through apoptosis induced by p21 which may relate with overexpression of tTG.

关 键 词：P21基因 基因转移 基因治疗 组织型转谷氨酰胺酶 脱噬作用 血管平滑肌细胞 细胞增殖 

分 类 号：R540.2[医药卫生—心血管疾病]