硒对胎鼠肝细胞核酸及蛋白质合成的影响  

THE EFFECT OF SELENIUM ON THE NUCLEIC ACID AND PROTEIN SYNTHESIS OF THE CULTURING FETAL RAT LIVER CELLS

作　　者：阎小君 贾锡安[2] 李广元 任瑛云 

机构地区：[1]中西医结合研究所 [2]西安医科大学生物化学教研室

出　　处：《西安医科大学学报》1991年第1期10-13,共4页Journal of Xi'an Medical University(Chinese)

基　　金：国家自然科学基金

摘　　要：本文报道了微量元素硒对培养纯种Wistar大白鼠胎肝细胞核酸及蛋白质合成的影响。观察了肝细胞培养基中不同硒水平对~3H-TdR(~3H-脱氧胸苷)、^(14)C-UR(^(14)C-尿苷)参入率的影响,同时还测定了培养基中白蛋白及甲胎蛋白(AFP)的含量。结果显示:细胞培养基中硒(Se)浓度在0.4～0.6μg/ml范围内使~3H-TdR、^(14)C-UR的参入率、白蛋白及甲胎蛋白含量均明显高于不补硒组。说明硒在这一浓度范围内能促进肝细胞DNA、RNA更新率及蛋白质合成。In order to determine the effect of trace elementselenium on dcoxyribonucleic acid (DNA), ribonucleicacid (RNA) and protein synthesis in culturing fetal rathepatocytes, the incroporation of ~3H-deoxythymidine(~3H-TdR) into DNA, ^(14)C-uridine (^(14)C--UR) intoRNA by fetal rat hepatocytes cultured inselenium--supplemented culture medium were investigated In addition the amounts of albumin and fetalprotein released from the same hepatocytes into the cul-ture medium were also determined. It was shown thatthe incorporation rate of ~3H--TdR into DNA and^(14)C--UR into RNA by fetal hepatocytes cultured in themedium supplemented with selenium as selenite at arang of Q 4 to Q 6 μg per ml levels were significantlyhigher than those cultured in medium withoutselenium--supplementation, concomitantly the amountsof albumin and--fetal protein released into the sameselenium--supplemented culture medium were alsogreater signicantly than those did in the mediumwithout selenium--supplementaion (as control). Theresults suggest that selenium supplementation in culturemedium at the levels of Q 4 to Q 6 μg selenium per mlcan promote the synthesis of DNA, RNA and proteinby fetal rat hepatocytes

关 键 词：硒 肝细胞 核酸 蛋白 

分 类 号：R333.4[医药卫生—人体生理学]