温度对水稻谷氨酰胺合成酶和NADH-谷氨酸合酶表达的影响  

Effect of Temperature on Expression of Glutamine Synthetase and NADH-Glutamate Synthase in Rice Plants

作　　者：陆彬彬[1] 周 卫[1] 张 吉[1] 潘 磊[1] 林清华[1] 张楚富[1] 

机构地区：[1]武汉大学植物发育生物学教育部重点实验室,湖北武汉430072

出　　处：《武汉大学学报（理学版）》2002年第2期239-242,共4页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金(39870527);国际合作基金资助项目

摘　　要：测定了不同温度（32℃和23℃）培养下水稻幼苗根和叶的谷氨酰胺合成酶（GS）同工酶以及依赖于NADH的谷氨酸合酶（NADH-GOGAT）的活性变化.结果表明,温度对水稻根和叶的GS活性具有相反的作用.23℃下生长的水稻根的GS活性明显高于32℃下生长的活性,而23℃下生长的叶的GS活性显著低于生长在32℃下的叶的活性.Native-PAGE和活性染色以及蛋白质印迹表明,根和叶的GS活性变化主要是由于GSrb和GS2活性及其相应蛋白质水平变化所致.23℃下生长的水稻根的NADH-GOGAT活性显著高于32℃下生长的活性,而叶的活性则基本保持恒定.We compared the activity of Glutamine Synthetase (GS) and NADH-Glutamate Synthase (NADH-GOGAT) from roots and leaves of rice plants cultured under different temperature C32℃ and 23℃) , respectively. The results showed that both GS and GOGAT activities of roots from rice grew under 23℃ were obviously higher than those of grew under 32℃ , but GS activity of leaves decreased a lot, whde GOGAT activity only decreased a little. Native-PAGE combined with activity staining and immunoblotting revealed that changes of GS activity in the roots and leaves may be mainly contributed to changes of GSrb and GS2.

关 键 词：谷氨酰胺合成酶 NADH-谷氨酸合酶 同工酶 水稻 温度 酶活性 活性染色 蛋白质印迹 

分 类 号：S511[农业科学—作物学] Q946.5[生物学—植物学]