棉花黄萎病菌毒素的分离提纯及致萎活性测定  

Isolation and Purification of Cotton Verticillium Wilt Pathogen and the Bioassay of wilting Phytotoxin

作　　者：甘莉 吕金殿 

机构地区：[1]陕西省植物保护研究所

出　　处：《西北农业大学学报》1991年第1期49-52,共4页Journal of Northwest Sci-Tech University of Agriculture and Forestry（Natural Science Edition)

摘　　要：经超滤,Con.A—Scpharosc 4B亲和层析,从棉花黄萎病菌的Czapck′s培养液中,分离纯化致萎毒素。成分分析和致萎活性测定结果表明:棉花黄萎病菌产生的毒素是一种致萎力极强的糖蛋白。其含糖量为14.74％,蛋白质量为85.26％,其致萎活力是半纯化液的10～15倍,活力回收为76％。The wilting toxin was separated and purified from Czapck's medium liquid of cotton vcrlicillium wilt pathogen by ultrafiltration and Con.A-Scpharosc 4B affinity labeling. The component analysis and the results from bioassay of wilting phytotoxin showed that toxin was a kind of glyco-protein with a strong wilting ability, which contains 14.74% of carbonhydratc and 85.26% of protein .The toxin wilting activity was 12-15 times higher than that of half crude toxin , and the retraction wilting activity was 76%.

关 键 词：棉花 黄萎病菌 致萎毒素 糖蛋白 

分 类 号：S435.621.2[农业科学—农业昆虫与害虫防治]