氯化镧拮抗内毒素作用的初步研究  

Antiendotoxic Effect of Lanthanum Chloride

作　　者：汪泱[1] 袁铿[2] 曹勇[1] 李国辉[1] 周水莲 戴育成[2] 

机构地区：[1]江西医学院第一附属医院烧伤研究所,江西南昌330006 [2]江西省医学科学研究所,江西南昌330006

出　　处：《中国稀土学报》2002年第2期190-192,共3页Journal of the Chinese Society of Rare Earths

基　　金：国家自然科学基金资助项目 ( 398690 0 3)

摘　　要：用定量鲎基质显色法测定LaCl3结合内毒素 (LPS)的能力。通过ELISA、荧光定量RT PCR方法观察LaCl3对LPS诱导的小鼠腹腔巨噬细胞 (Mφ)肿瘤坏死因子 α(TNF α)分泌及TNF αmRNA表达的影响。通过小鼠死亡率观察LaCl3对LPS毒性的影响。结果显示LaCl3具有结合LPS ,抑制TNF α分泌及TNF αmRNA表达 。The ability of LaCl 3 to bind lipopolysaccharide (LPS) was determined based on quantitative limulus test. The effect of LaCl 3 on the secretion of TNF α and the expression of TNF αmRNA in murine peritoneal macrophages stimulated by LPS was studied by ELISA and SYBR Green I fluorescence quantitative RT PCR method. The influence of LaCl 3 on the virulence of endotoxin was evaluated by observing the mortality rate of mouse challenged by LPS. The results indicate that LaCl 3 has functions of binding LPS, decreasing the toxicity of LPS, suppressing secretion of TNF α and expression of TNF αmRNA.

关 键 词：稀土 氯化镧 内毒素 

分 类 号：Q936[生物学—微生物学] R630.3[医药卫生—外科学]