利钾尿肽放射免疫分析法及初步应用  

Radioimmunoassay of kaliuretic peptide

作　　者：曾强[1] 范英鲜[1] 郑秋甫[1] 

机构地区：[1]解放军总医院南楼二科,北京100853

出　　处：《中华核医学杂志》2002年第2期122-124,共3页Chinese Journal of Nuclear Medicine

基　　金：国家自然科学基金资助项目 (39970 80 6);全军九五医药卫生科研基金资助项目 (98Q0 92 )

摘　　要：目的　建立利钾尿肽 (KP)放射免疫分析法 (RIA)并用于临床。方法　将KP和甲状腺球蛋白连接制成免疫原 ,免疫新西兰白兔获得高特异性KP抗体 ,采用Iodogen法制备12 5I KP ,经高效液相色谱纯化后其比活度为 6 4.75TBq/mmol。测定尿毒症、高血压、急性心肌梗死患者及正常对照者的血浆KP含量。结果　方法的测定范围是 5 0～ 30 0 0pmol/L ,IC50 =92 5pmol/L。对照组血浆KP含量为 (2 6 0 82± 2 9 45 )pmol/L ,而尿毒症、高血压、急性心肌梗死患者的KP含量均显著升高。结论　KPRIA的建立为开展相关研究提供了一种灵敏。Objective To develop a sensitive and specific radioimmunoassay of kaliuretic peptide (KP) and apply it in clinical research. Methods KP and thyreoprotein were conjugated and the conjugate was injected into New Zealand white rabbits. A high sensitive and specific antibody of KP was obtained. 125I-KP was prepared by Iodogen method and was purified by HPLC. Its specific activity was about 64.75 TBq/mmol. The plasma concentrations of 125I-KP of the healthy volunteers and the hypertensive, uraemia and acute myocardial infarction patients were determined. Results The assaying range was 50～3 000 pmol/L, IC 50=925 pmol/L. The plasma KP value of the healthy volunteers was (260.82±29.45) pmol/L (n=25) and the KP levels in the hypertensive, uraemia and acute myocardial infarction patients were significantly higher than that in normal subjects. Conclusion This developed assay could be a usable technique in related research of KP.

关 键 词：利钾尿肽 放射免疫分析 尿毒症 心肌梗塞 

分 类 号：R817[医药卫生—影像医学与核医学]