不同方式标记非放射性探针原位杂交效果评价  

The in situ hybridization effort estimation between antisense RNA probe and random primed DNA probe

作　　者：刘宁国[1] 赵子琴[2] 顾云菊[2] 陈忆九[1] 阎祖康[2] 廖燕萍[2] 

机构地区：[1]司法部司法部司法鉴定科学技术研究所,上海200063 [2]复旦大学基础医学院法医学系,上海200032

出　　处：《法医学杂志》2002年第1期7-8,共2页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(No:39770816)

摘　　要：目的比较不同方式标记非放射性探针的原位杂交效果。方法通过两种方法标记地高辛随机引物DNA探针和反意RNA探针,在大鼠损伤模型的皮肤组织上进行原位杂交。结果两种探针均可满足要求,以反意RNA探针在阳性染色深度和背景方面效果更佳。结论RNA探针在杂交效果方面优于DNA探针。Objective To estimate the effort between antisense RNA probe and random primed DNA probe. Methods The in situ hybridization was conducted on parafin section from wounding model of rat skin. Results Although both probes appeared positive staining, RNA probes was superior to DNA probes in terms of depth of staining and background. Conclusion RNA probe showed more satisfactorily on ISH.

关 键 词：原位杂交 地高辛 随机引物探针 反意RNA探针 法医病理学 

分 类 号：D919.1[医药卫生—法医学]