温州蜜柑萎缩病毒的提纯法研究  

STUDIES ON PURIFICATION TECHNIQUES FOR SATSUMA DWARF VIRUS

作　　者：周常勇[1] 赵学源[1] 蒋元晖[1] 龚祖埙[2] 吴建华[2] 陈作义[2] 

机构地区：[1]中国农业科学院柑桔研究所 [2]中国科学院上海生化研究所

出　　处：《西南农业大学学报（自然科学版）》1991年第1期92-95,共4页Journal of Southwest Agricultural University

摘　　要：本文提出的温州蜜柑萎缩病毒(SDV)提纯法,仅用100～200克病洋酸浆叶,且无需通过蔗糖密度梯度离心,在电镜下亦能看到中量直径约25nm 的球状病毒颗粒,粗提纯制剂OD_(860)/OD_(280)平均值为1.28,OD_(260)/OD_(240)平均值为1.15,初步显示出SDV 特征性紫外吸收曲线,提纯病毒产量约为4.5～11mg/kg病叶。比较证明,0.01M、PH7.0和0.1M、PH6.6的柠檬酸缓冲液以及0.5M、PH7.0的磷酸缓冲液是适宜的抽提缓冲液,洋酸浆是最好的繁殖寄主。An improved purification procedure for satsuma dwarf virus (SDV)without sucrose density-gradient centrifugation was developed,by whichglobular SDV particles about 25 nm in diameted were observed with elec-tron microscopy,using only 100-200 g of Physalis floridana-infectedleaves as the virus source.The purufied SDV showed that the ratios ofOD260/OD280 and OD260/OD240 were 1.28 and 1.15,respectively.Theyield of purified SDV ranged from 4.5 to 11 mg/kg infected leaves.Citrate buffer(0.01M,pH7.0 or 0.1M,pH6.6)and phosphate buffer(0.5M,pH7.0) were shown to be preferable for SDV purification and P.floridanais a good propagative host for SDV.

关 键 词：温州蜜柑 萎缩病毒 提纯法 柑桔 

分 类 号：S436.661.1[农业科学—农业昆虫与害虫防治]