彗星试验非荧光染色研究  

Studies on Un-fluorescent Staining Methods in Comet Assay

作　　者：赵蓉[1] 衡正昌[1] 

机构地区：[1]四川大学华西公共卫生学院环境卫生教研室,成都610041

出　　处：《环境与健康杂志》2002年第2期138-140,共3页Journal of Environment and Health

基　　金：国家自然科学基金资助 (39670625)

摘　　要：目的为了建立一种非荧光染色的彗星试验。方法将Feulgen法、甲基绿染色法、全染料 (stains_all)法和银染法等几种非荧光DNA特异染色方法试用于彗星试验 ,并与溴化乙锭 (EB)染色进行了比较。结果在所试验的染色法中 ,仅银染法有满意的效果 ,染色清晰 ,“彗星”头和尾的边界易于判断 ,染色持久 ,敏感性高 ,可染出EB不能染出的DNA小片段 ,得到“彗星”头长和尾长均为EB染色的2倍以上 ,安全、价廉。结论银染法摒弃了EB染色的不足 ,在彗星试验中可以替代EB染色 ,为彗星试验的进一步推广提供了可能。Objective To develop an un_fluorescent staining method in Comet Assay. Methods Some special un_fluorescent DNA staining methods, Feulgen, Methyl Green, Stains_all and Silver_Stain were tested in Comet Assay and the comparison studies with EB stain were carried out. Results Among the stain methods tested, just the silver_stain could give a satisfactory effect: legible, ease to judge the stained area of the comets head and tail, permanent, sensitive, could make little segment of DNA be stained, which couldnt be stained by EB stain, the length of comets head and tail, were over 2 times of that of EB stain, safety and economical. Conclusion The silver_stain method discarded the defects of EB stain, so that it could replace EB stain and make comet assay possible to be extended.

关 键 词：DNA损伤 实验室诊断 彗星试验 银染法 

分 类 号：R994.6[医药卫生—毒理学] R361[医药卫生—药学]