CB-HRP—高效敏感的神经束路追踪方法  

CB-HRP—A HIGH SENSITIVITY METHOD TO NERVE TRACT TRACING

作　　者：张晓[1] 高礼[1] 欧可群[2] 杨淑霞[1] 

机构地区：[1]第三军医大学成都军医学院实验技术教研室,成都610083 [2]四川大学华西医学院组胚教研室,成都610041

出　　处：《西南国防医药》2002年第2期100-102,共3页Medical Journal of National Defending Forces in Southwest China

基　　金：成都军医学院院长基金资助 NO :2 0 0 1 0 0 1 2

摘　　要：目的 :寻找一种高效敏感的神经通路追踪方法。方法 :采用结合了霍乱毒素亚单位B的辣根过氧化物酶 (CB -HRP)作为示踪剂 ,用钨酸钠作为四氨基联苯 (TMB)显色剂的稳定剂 ,顺行追踪视神经纤维向下丘脑前区的投射 ;逆行追踪舌下神经纤维在延髓背侧的分布。结果 :发现视神经纤维投射到视交叉上核背内侧 ;舌下神经纤维在延髓背侧的舌下神经纤维核分布区显示神经元胞体和轴突结构清晰。结论 :用霍乱毒素亚单位B与辣根过氧化物酶结合作为示踪剂 。Objective:To find a high sensitivity method of nerve tract tracing with horseradish peroxidase. Method:Horseradish peroxidase was conjugated with cholera toxin (CB-HRP) as the tracing agent, and sodium tungstae was used as the stabilizer of CB-HRP. The agent was used to trace theoptic nerve fibers projecting to the area of the hypothalamus and hypoglossus nerve fibers distributing to the dorsal area of the medulla oblongate. Result:It was showed that the optic nerve fibers projected to the suprachiasmatic nucleus, to the lateral hypothalamus as well as the peri-part of the supraoptic nucleus. The hypoglossus nerve nucleus were seen in the dorsal portion of the medulla oblongate. Conclusion:This method is a high sensivity method to nerve tract tracing.

关 键 词：辣根过氧化物酶 钨酸钠 神经示踪 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]