大鼠严重烧伤早期大脑皮质损伤机制的实验研究  

Study on potential mechanism of cerebral cortex damage in rats following severe burns

作　　者：王水明[1] 刘友生[1] 陈锐[1] 

机构地区：[1]第三军医大学西南医院病理学研究所,重庆400038

出　　处：《中国危重病急救医学》2002年第2期75-77,共3页Chinese Critical Care Medicine

基　　金：国家重点基础研究发展规划项目 ("973"项目 )子课题( No.G19990 5 42 0 2 )

摘　　要：目的 :探讨大鼠严重烧伤早期大脑皮质的损伤特点及其发生机制。方法 :采用光、电镜观察大鼠 30 %总体表面积 (TBSA) 度烧伤后 2 4小时内大脑皮质的病理形态学改变 ,同时对大脑皮质 ATP酶、一氧化氮(NO)、黄嘌呤氧化酶 (XO)、髓过氧化物酶 (MPO)及丙二醛 (MDA)的含量或活性进行动态观测。结果 :大鼠严重烧伤后 3小时即出现皮质疏松、细胞水肿和血管外间隙增宽 ,其后病变加重并出现神经元灶性溶解。 3种ATP酶活性在伤后呈进行性降低 ;NO和 XO在伤后 1～ 2 4小时、MPO在伤后 6～ 2 4小时、MDA在伤后 3～2 4小时均显著高于正常对照组 ,XO、MPO分别与 MDA呈显著正相关。结论 :严重烧伤早期大脑皮质即可出现一系列显著病理性损害 ,缺血缺氧、NO产生、XO活化、中性粒细胞浸润及脂质过氧化在大脑皮质损伤中发挥着重要作用。Objective:To explore damage of cerebral cortex following severe burns and its potential mechanism.Methods:An animal model in which rats were subjected to 30% total body surface area(TBSA)Ⅲ degree burns was used for this experiment,dynamic changes in activities or contents of ATPase,nitric oxide(NO),xanthine oxidase(XO),myeloperoxidase (MPO),melondialdehyde(MDA) were determined, and pathological changes in the cerebral cortex were also measured by light and electron microscopy and spectrophotography.Results:Rarefaction of cortex,edema of cells,broaden of interspace around blood vessel were found at 3 hours post burn,pathological changes were progressively aggravated and focal lysis of neuron appeared thereafter. ATPase activities persistently decreased.There were significantly differences in activities or contents of NO,XO,MPO and MDA between normal control and burn group at 1 to 24,6 to 24,3 to 24 hours after severe burns,respectively.In addition,activities of XO and MPO were positively correlated with contents of MDA.Conclusions:It is demonstrated that early pathological lesion could be shown in cerebral cortex after severe burn,hypoxiaischemia,NO induction,activation of XO,infiltration of neutrophils and lipid peroxidation may play important roles in the development of cerebral cortex damage.

关 键 词：大脑皮质损伤 烧伤 大鼠 病理形态学变化 实验研究 

分 类 号：R644[医药卫生—外科学]