乌司他丁对急性坏死型胰腺炎大鼠肺损伤的保护作用  

Protective effects of ulinostatin on acute lung injury induced by acute necrotizing pancreatitis in rats

作　　者：尹勇[1] 高乃荣[2] 李兆丽 

机构地区：[1]广州中山医科大学附属第一医院胃肠外科,广东省广州市510089 [2]东南大学医学院附属中大医院普外科,江苏省南京市210009 [3]佛山市第一人民医院病理科,广东省佛山市528000

出　　处：《世界华人消化杂志》2002年第5期558-561,共4页World Chinese Journal of Digestology

摘　　要：目的:观察乌司他丁(VT)对急性坏死型胰腺炎(ANP)大鼠肺损伤的保护作用。 方法:SD大鼠72只,随机分为3组:假手术组(SO);ANP组;ANP+UT组(10万U/kg)。通过结扎大鼠十二指肠使呈闭袢制备ANP模型。免疫组织化学法观察ICAM-1蛋白的表达,测定肺脏髓过氧化物酶(MPO)活性,同时观察术后6h肺毛细血管通透性(LCP)的变化及肺组织的病理形态学改变。 结果:UT治疗组肺脏ICAM-1的表达下调,肺脏MPO活性虽明显高于SO组,但较ANP组明显降低(6h:3.0±1.0μmol/g us 46±0.8 μmol/g,P<0.05;16 h:3.5±1.1 μmol/g vs 7.7±0.7μmol/g,P<0.01;24h:3.3±1.3tμmol/g vs7.2±1.0μmol/g,P<0.01).LCP水平也较ANP组明显降低(74.9±6.6μmol/g vs vs 86.9±10.9μg/g ,P<0.05),肺损伤程度较ANP组明显减轻。结论:UT通过下调肺脏ICAM-1的表达,减少中性粒细胞在肺脏的聚集、浸润,对于急性坏死型胰腺炎大鼠的肺损伤具有一定保护作用。AIM:To investigate the protective effects of ulinostatin (UT) on acute lung injury in acute necrotizing pancreatitis (ANP) in rats.METHODS: Seventy-two Sprague-Dawley rats were randomly divided into three groups: sham operation (SO) group, ANP group, ANP + UT group (UT: 100 KU/kg). ANP model was made by creating a closed duodenal loop in rats. Pulmonary ICAM-1 expression was detected by using ABC immunohistocrtemistry and the accumulation of polymorphonuclear leukocytes in lung tissue was determined by the myeloperoxidase (MPO) assay. Lung capillary permeability (LCP) and pathological changes of rat lung tissue were also observed.RESULTS: UT treatment markedly downregulated ICAM-1 expression and pulmonary MPO activity of UT group was significantly decreased as compared with that of ANP group(6h: 3.0±1.0nmol/g vs 4.6±0.8μmol/g, P<0.05; 16 h: 3.5±1.1μmol/g vs7.7±0.7μmol/g, P<0.01; 24h: 3.3± 1.3μmol/g vs 7.2±1.0μmol/g, P<0.01), although it was still higher than that of SO group. LCP level of UT group was reduced as compared to ANP group(74.9 ± 6.6 μg/g vs 86.9 ± 10.9 μg/g, P < 0.05). Pathological changesof lungtissue revealed that lung injury was significantly improved with UT treatment.CONCLUSION: Ulinostatin can reduce the accumulation and infiltration of polymorphonuclear leukocytes in lung tissue via downregulating of ICAM-1.Thus, this drug may exert a beneficial effect on acute lung injury induced by acute pancreatitis.

关 键 词：乌司他丁 急性坏死型胰腺炎 大鼠 肺损伤 保护作用 

分 类 号：R657.5[医药卫生—外科学]