胎盘组织中诱导型一氧化氮合酶和巨噬细胞的表达与妊娠高血压综合征发病的关系  

Expression of inducible nitric oxide synthase and macrophage in human placenta of pregnancy induced hypertention syndrome

作　　者：左建新[1] 张陵娜[2] 曾文洁[2] 

机构地区：[1]青岛大学医学院附属医院妇产科,266003 [2]中南大学湘雅医院妇产科

出　　处：《中华妇产科杂志》2002年第6期331-334,共4页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的　通过测定妊娠高血压综合征 (妊高征 )孕妇胎盘组织中诱导型一氧化氮合酶和巨噬细胞的表达 ,探讨其在妊高征发病中的作用。方法　应用免疫组织化学方法检测 30例妊高征孕妇 (妊高征组 ,其中轻度 2例 ,中度 7例 ,重度 2 1例 )和 2 2例正常妊娠妇女 (对照组 )胎盘组织中诱导型一氧化氮合酶和巨噬细胞的表达。结果　 (1)妊高征组孕妇胎盘组织中诱导型一氧化氮合酶和巨噬细胞表达的阳性率分别为 (2 9± 0 7) %和 (3 0± 0 8) % ,对照组孕妇分别为 (2 1± 0 8) %和 (2 1±0 7) % ,两组比较 ,差异有极显著性 (P <0 0 1)。 (2 )轻、中度妊高征孕妇胎盘组织中诱导型一氧化氮合酶和巨噬细胞表达的阳性率分别为 (2 8± 0 7) %和 (2 8± 1 0 ) % ,重度妊高征孕妇分别为 (3 0±0 7) %和 (3 1± 0 8) % ,两者之间比较 ,差异无显著性 (P >0 0 5 )。结论　妊高征患者胎盘组织中诱导型一氧化氮合酶和巨噬细胞均有高表达 ,这可能是引起胎盘功能不良和胎儿 胎盘循环阻力改变的原因之一 。Objective To investigate the expression of inducible nitric oxide synthase(iNOS) and macrophage(CD 68 ) in the placenta of the patients with pregnancy induced hypertention syndrome(PIH) and the role of them in the pathogenesis of PIH Methods Immunohistochemical technique (SABC) was used for immunostaining and detecting inducible nitric oxide synthase and CD 68 in the placentas of thirty patients of PIH (two patients of mild PIH; seven moderate PIH; twenty one severe PIH) and twenty two normal pregnancies (the control group) Results The level of inducible nitric oxide synthase and CD 68 in the placenta of patients of PIH were(2 9±0 7)% and(3 0±0 8)% ,respectively and (2 1±0 8)% and (2 1±0 7)% in the control group They were both significantly different ( P <0 01) The levels of inducible nitric oxide synthase in the mild morderate and severe PIH were (2 8±0 7)% and (3 0±0 7)%,and the CD 68 were (2 8±1 0)% and (3 1±0 8)%, which were not significantly different ( P >0 05) Conclusion The obvious increase of iNOS and macrophage in the placenta of patients of PIH can be observed and iNOS and CD 68 are involed in the immunization and inflammatory reaction This may be one of the causes for human placental dysfunction and high impedance of fetoplacental circulation in PIH It may play an important role in the genesis of pregnancy induced hypertention

关 键 词：胎盘 一氧化氮合酶 巨噬细胞 妊娠高血压综合征 相关性 免疫组织化学 

分 类 号：R714.246[医药卫生—妇产科学]