尿素包接法预浓缩α-维生素E的试验研究  

Preconcentration of α-Vitamin E Using Urea Inclusion Method

作　　者：樊明涛[1] 吴守一[1] 马海乐 

机构地区：[1]江苏大学

出　　处：《农业工程学报》2002年第2期106-109,共4页Transactions of the Chinese Society of Agricultural Engineering

基　　金：江苏省自然科学基金资助项目 (BK99112 )

摘　　要：豆油脱臭馏出物含有丰富的维生素 E和大量的游离脂肪酸 ,是浓缩天然维生素 E的优质原料 ,但由于游离脂肪酸和维生素 E在超临界 CO2 中具有相似的溶解度 ,用超临界 CO2 萃取难以提高维生素 E的浓度。尿素包接可除去脱臭馏出物中的大部分饱和游离脂肪酸 ,为超临界 CO2 萃取提供良好原料。试验结果表明 ,尿素包接法预浓缩维生素 E的效能主要和脱臭馏出物与尿素的摩尔比、溶解尿素的甲醇或乙醇的浓度有关 ,两种试剂溶解尿素表现了相似的包接效果 ,回流时间和温度对包接效果基本无影响。包接的最佳条件为 ,豆油脱臭馏出物与尿素的摩尔比为 1∶ 15 ,溶剂为 95 %的乙醇 ,6 0℃回流 10 min,1～ 2℃静置 6～ 8h,过滤除去包接物。尿素包接可使剩余物中α-维生素 E的浓度从原料的 0 .5 7%上升到 1.6 2 % ,包接物中含有较多与维生素 E有相似溶解度的游离脂肪酸 ,而α-维生素 E的浓度仅为 0 .1%～ 0 .2 %。以剩余物为原料 ,利用超临界 CO2 萃取可将 α-维生素 E的浓度提高到5 .6 %以上。Soybean deodorized sludge (SDS) contains rich tocopherols and large amounts of free fatty acids and is good resource to concentrate vitamin E, but free fatty acids and tocopherols have similar solubilities in supercritical CO 2, so it is very difficult to increase tocopherols concentration by supercritical CO 2 extraction. Urea inclusion can remove most of saturated fatty acids from SDS and provide better material for supercritical CO 2 extraction. The results showed that the molar ratios of SDS to urea and water content in solvent had remarkable effect on compositions in inclusion part and filtrate, refluxing time and temperature had little influence on urea inclusion compositions in the two parts, methanol and ethanol showed similar action when they were used as urea solvent. The best inclusion conditions are as follows: the molar ratios of SDS to urea were 1∶15,95% ethanol as solvent to dissolve urea, refluxing for 10 min at 60 ℃ and then the solution was kept at 1～2 ℃ for 6～8 h.Through urea inclusion, α vitamin E could be increased from 0 57% in SDS to 1 62% in filtrate, while inclusion part contained large amounts of fatty acids with similar solubility in supercritical CO 2,and only 0 1%～0 2% α vitamin E. Using filtrate as material to reconcentrate α vitamin E by supercritical CO 2, the concentration of α vitamin E can be increased to 5 6%.

关 键 词：豆油脱臭馏出物 α-维生素E 尿素包接法 预浓缩 

分 类 号：R977.25[医药卫生—药品]