土壤中硝化细菌的筛选及初步鉴定  

Screening and Preliminary Identification of Nitrifying Bacteria in Soil

作　　者：孙克江 郑金来[2] 

机构地区：[1]天津市东丽区卫生防病站,天津300300 [2]军事医学科学院卫生学环境医学研究所

出　　处：《环境与健康杂志》2002年第3期238-239,共2页Journal of Environment and Health

摘　　要：目的 为治理水环境氮污染提供科学方法。方法 通过富集、培养,在土壤中筛选出自氧型氨氧化菌,在进行形态鉴定后,又对其硝化速率进行测定。结果 所筛选出的12种菌株革兰染色均阴性,短杆状为主。28℃培养7d后形成菌落,以直径小、圆形、淡黄色、光滑型为主。硝化速率为0.40mg/（L·d）～1.15mg/（L·d）。结论 所筛选的12种菌株均具有硝化功能,其中部分菌株可用于水环境氮污染的治理。ve To control and minimize the water pollution by nitrogen. Methods The autotrophic amino-oxidizing bacteria in soil were screened by enrichment and incubation. After bacterio-morphological identifica-tion their nitrifying rates were determined. Results All of the 12 screened strains were gram-negative and in the shape of brevi-rod dorminantly. The colonies with a dorminant form of short diameter in round shape, light yellow and smooth formed after 7-day incubation at 28℃ . The nitrifring rates ranged 0.40 mg/ (L·d)~1. 15 mg/ (L·d) . Conclusion The screened 12 strains all presented nitrifying function, some of them could be used to control water environment pollution by nitrogen.

关 键 词：细菌 硝化杆菌属 土壤 

分 类 号：S154.3[农业科学—土壤学]