乌头中剧毒生物碱的提取分离  

Separation of toxical aconitum alkaloids

作　　者：王朝虹[1] 何毅[2] 张继宗[2] 胡春华[2] 

机构地区：[1]北京大学药学院,100081 [2]公安部物证鉴定中心,北京100038

出　　处：《刑事技术》2002年第3期16-17,共2页Forensic Science and Technology

摘　　要：目的从乌头属植物中提取分离剧毒生物碱乌头碱、中乌头碱、次乌头碱;方法酸性水溶液提取,碱化后中性氧化铝柱层析,IR、EI-MS确认结构;结果利用该方法提取以上3种生物碱得到的回收率与碱性苯溶液法相似。结论该方法所用试剂成本低、毒性小,适用于制备乌头碱、中乌头碱、次乌头碱对照品。Objective To establish a method for extracting and separating mesaconitine,aconitine and hypaconitine.Methods Aconitum kusnezoffii reichb was extracted with0.2mol/L HCl and adjusted to pH10.The precipitate was chromatographed over neutral alumina.Each alkaloid's structure was identified with IR and EI-MS.Results The recovery received from this method was similar with that of ammoniacal benzene method.Conclusion This method was at low cost,harmless and suitable for preparing mesaconitine,aconitine and hypaconitine.

关 键 词：乌头 生物碱 提取 分离 中毒检验 

分 类 号：D919.4[医药卫生—法医学]