红平红球菌LSSE8-1的培养及其对二苯并噻吩中硫元素脱除的影响  

Growth and Specific Desulfurization of a Newly Isolated Strain of Rhodococcus erythropolis

作　　者：李珊[1] 邢建民[1] 缑仲轩[1] 罗明芳[1] 刘会洲[1] 陈家镛[1] 

机构地区：[1]中国科学院过程工程研究所分离科学与工程青年实验室及生化工程国家重点实验室,北京100080

出　　处：《过程工程学报》2002年第3期257-261,共5页The Chinese Journal of Process Engineering

基　　金：国家重点基础研究发展规划资助项目(编号: G2000048004)

摘　　要：红平红球菌(Rhodococcus erythropolis)LSSE8-1是一株新分离的专一性脱硫菌,HPLC分析表明该菌能选择性地脱除二苯并噻吩(DBT)中的硫,最终代谢产物是2(羟基联苯(2-HBP). 适宜的初始pH为6.5~9;能利用多种碳源,其中以甘油为最佳,适宜的甘油浓度为8 g/L;2 g/L的乙酸铵是菌体生长和脱硫的合适氮源;二甲基亚砜是提高该菌细胞收率和脱硫比活性的有效硫源.A newly isolated strain was identified as Rhodococcus erythropolis LSSE8-1, which can specifically remove sulfur from the model compound of dibenzothiophene (DBT) with 2-hydroxydiphenyl as the dead-end metabolic product. The optimal initial pH for growth varies from 6.5 to 7. Glycerol with the concentration of 8 g/L is the appropriate carbon source, and acetic ammonia at 2 g/L is suitable as nitrogen source for cell growth and desulfurization. It was found that R. erythropolis LSSE8-1 grew in the presence of sulfate or sulfur-containing amino acids but showed no desulfurization activity. Dimethylsulfoxide can enhance the cells yield and the activity of desulfurizing enzymes is not inhibited.

关 键 词：生物脱硫 二苯并噻吩 红平红球菌 石油产品 

分 类 号：TE624.1[石油与天然气工程—油气加工工程]