压电免疫传感器用于乙肝表面抗原的测定  

Development of a Piezoelectric Immunosensor for Hepatitis B Surface Antigen

作　　者：陈泽忠[1] 王柯敏[1] 陈永康[2] 肖丹[1] 羊小海[1] 邵国强[2] 

机构地区：[1]化学生物传感与计量学国家重点实验室,湖南大学化学化工学院长沙410082 [2]香港浸会大学化学系

出　　处：《高等学校化学学报》2002年第6期1044-1046,共3页Chemical Journal of Chinese Universities

基　　金：The Research Grants Council of the Hong Kong Special Adm inistration Region;China(No.HKBU 2 0 5 4/98p) ;国家杰出青年基金 (批准号 :2 982 5 110 );湖南省自然科学基金 (批准号 :0 0 GKY10 10 )资助

摘　　要：A piezoelectric immunosensor has been developed for the detection of Hepatitis B surface antigen(HBsAg). Treatment of a Au electrode with cystamine solution results in cysteamine self assemble monolayers(SAMs). Using a bifunctional cross linker, glutaraldehyde, the antibody was immobilized on the electrode surface. The influence factors such as the reaction time of immunoreaction and the concentration of antibody etc. were investigated. Under the optimized experimental conditions, a series standard concentration of HBsAg solutions were analyzed in solution. The frequency shift was linear with the antigen mass concentration in the range of 0 2-12 0 μg/mL. The analysis results of two serum specimens showed that the positive serum and the negative serum could be distinguished easily. Glycine HCl solution(0 2 mol/L) was used for desorption of the bound antigen. The crystal could be regenerated nearly 8 times without detectable loss of activity.A piezoelectric immunosensor has been developed for the detection of Hepatitis B surface antigen(HBsAg). Treatment of a Au electrode with cystamine solution results in cysteamine self assemble monolayers(SAMs). Using a bifunctional cross linker, glutaraldehyde, the antibody was immobilized on the electrode surface. The influence factors such as the reaction time of immunoreaction and the concentration of antibody etc. were investigated. Under the optimized experimental conditions, a series standard concentration of HBsAg solutions were analyzed in solution. The frequency shift was linear with the antigen mass concentration in the range of 0 2-12 0 μg/mL. The analysis results of two serum specimens showed that the positive serum and the negative serum could be distinguished easily. Glycine HCl solution(0 2 mol/L) was used for desorption of the bound antigen. The crystal could be regenerated nearly 8 times without detectable loss of activity.

关 键 词：测定 压电免疫传感器 表面抗原 自组装膜 乙型肝炎 诊断 

分 类 号：R512.62[医药卫生—内科学] R446.62[医药卫生—临床医学]