稻秆木素侧链^(13)C同位素示踪及固体^(13)CNMR分析  

Carbon-13 Enrichment of Rice Stalk Lignin Traced by Solid State ^(13)C NMR Spectroscopy

作　　者：顾瑞军[1] 谢益民[1] 曾绍琼[1] 伍红[1] 安田征市[2] 

机构地区：[1]华南理工大学制浆造纸工程国家重点实验室,广州510640 [2]日本名古屋大学农学部

出　　处：《高等学校化学学报》2002年第6期1073-1076,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 99740 11);霍英东教育基金 (批准号 :710 66);广东省千百十工程优秀人才基金资助

摘　　要：在水稻 (Oryza sativa L.)生长过程中 ,向其茎秆部节间的空腔分别注入在侧链α,β,γ位带有 1 3C标记的松柏醇葡萄糖甙 ,得到 1 3C标记的稻秆木素 ,用高分辨率固体核磁 1 3C NMR对其组织进行分析 ,发现外源性的松柏醇葡萄糖甙并不影响水稻中木素的正常合成 .证明了 β-O-4,β-β,β-5和 β-1结构是稻秆原本木素中的主要结构 ,另外还有少量的松柏醇和阿魏酸类结构 ,并证实木素在Carbon 13 enrichment of specific carbon of rice stalk lignin was achieved by feeding coniferin [ α 13 C], coniferin [ β 13 C] and coniferin [ γ 13 C] into an internode cavity of the stalk of a growing rice plant( Oryza sativa L.). The resulting rice stalk tissues were analyzed by high resolution solid state carbon 13 nuclear magnetic resonance ( 13 C NMR) spectroscopy. It was found that the administration did not affect the normal ligninfication of rice plant. It was also proved that β O 4, β β, β 5 and β 1 were mainly a substructure in protolignin of rice stalk although there were some minor substructures as coniferyl alcohol and ferulic acid unit. Importantly, the result indicated that significant bonding occurred at α position to other constituents, presumed to be mainly of carbohydrates.

关 键 词：稻秆 木素 同位素示踪 碳13 核磁共振 分离 造纸工业 制浆 

分 类 号：TS74[轻工技术与工程—制浆造纸工程] O636.2[理学—高分子化学]