蝎毒对白血病HL-60细胞生长的抑制和诱导凋亡作用研究  

Studies of Buthus martensii Karsch (BMK) on inhibition of cell growth and induction of apoptosis in leukemic HL-60 cell line

作　　者：贾莉[1] 孟秀香[1] 孙宏丹[1] 宋振岚[1] 孔力[2] 魏巍[3] 

机构地区：[1]大连医科大学检验系临床血液教研室,大连116027 [2]大连医科大学检验系临床组胚教研室,大连116027 [3]大连医科大学检验系临床生化教研室,大连116027

出　　处：《中药药理与临床》2001年第6期26-28,共3页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的 :研究蝎毒对HL 60细胞的生长抑制和诱导凋亡作用 ,并探讨其作用机制。方法 :应用MTT法观察蝎毒对细胞的抑制作用 ,光镜、荧光显微镜和透射电镜观察细胞结构的改变 ,原位末端标记检测DNA断裂 ,流式细胞术分析凋亡细胞和bcl 2蛋白表达。结果 :蝎毒可明显地抑制HL 60细胞生长和诱导细胞凋亡 ,作用在一定范围内呈对浓度和时间的依赖性 ,蝎毒并能下调HL 60细胞bcl 2蛋白表达。结论 :蝎毒能抑制HL 60细胞生长和诱导细胞凋亡 ,其作用机制可能与bcl 2蛋白表达的下降有关。To study the mechanism of BMK on inhibition of cell growth and induction of apopsis in HL 60 cell line. Methods: The growth inhibition was analysed by M TT assay, cell apoptosis by light microscopy, fluorescence microscopy, transmiss ion electron microscopy, flow cytometry and TUNEL technique, the expression of b cl 2 protein was analysed by flow cytometry. Results: The results showed that BMK could inhib it cell growth and induce apoptosis obviously, and the effect of BMK was dose an d time dependent within a limited range. Flow cytometry showed that BMK could in hibit the expression of bcl 2 protein. Conclusion: BMK could inhibit HL 60 cell line g rowth and induce apoptosis, and the mechanism of effect occured through the expr ession of bcl 2 protein.

关 键 词：蝎毒 细胞凋亡 HL-60细胞 BCL-2基因 白血病 

分 类 号：R285.5[医药卫生—中药学] R273.37[医药卫生—中医学]