伸展态β-乳球蛋白重折叠过程的动力学特性  

THE KINETIC CHARACTERISTIC OF UNFOLDED β-LACTOGLOBULIN IN REFOLDING PROCESS

作　　者：萨楚尔夫[1] 罗辽复[2] 

机构地区：[1]内蒙古师范大学物理系,内蒙古呼和浩特010022 [2]内蒙古大学理工学院物理系,内蒙古呼和浩特010021

出　　处：《内蒙古师范大学学报（自然科学汉文版）》2002年第2期108-111,共4页Journal of Inner Mongolia Normal University(Natural Science Edition)

基　　金：国家自然科学基金资助项目 (3996 0 0 2 3)

摘　　要：从蛋白质变性的渐变模型及构象态渐变的观点出发 ,对伸展态 β -乳球蛋白的重折叠过程进行了分析 ,发现蛋白质分子从伸展态到最终折叠态历经一系列中间稳定态 .尿素浓度梯度电泳实验结果显示 ,存在两条不同的折叠途径 .在不同的尿素浓度范围内 。Starting from the viewpoint on sequential alternation of conformations,the refolding processes of the unfolded β lactoglobulin is analyzed.We find that the unfolded protein molecules undergo a series of steady intermediate states from unfolded conformation to the more intact conformation or native conformation.The experimental results of urea gradient electrophoresis show that there are two different refolding processes.In different regions of urea concentrations,the folding processes indicate that different processes correspond to different kinetic features.

关 键 词：伸展态 Β-乳球蛋白 重折叠过程 蛋白质折叠 中间稳定态 动力学特性 折叠机理 折叠动力学 

分 类 号：Q512.2[生物学—生物化学] Q615