小鼠胚胎计数方法的研究及应用  

Study of Counting Method of Mouse Embryo and its Application

作　　者：孙兴参[1] 伊亚玲[1] 岳奎忠[1] 贺桂馨 谭景和[1] 秦鹏春 

机构地区：[1]东北农业大学生命科学学院组织胚胎研究室,黑龙江哈尔滨150030

出　　处：《黑龙江畜牧兽医》2002年第6期3-5,共3页Heilongjiang Animal Science And veterinary Medicine

摘　　要：胚胎细胞计数是评定胚胎活力,检验胚胎体外操作方案和培养效果的常用方法。实验以昆明小鼠体内发育胚胎和体外聚合囊胚为实验材料,对几种细胞的计数方法及应用进行了详细的研究。结果表明:（1）低渗压片法的实验效果最佳。（2）根据形态学观察,结合细胞计数,可以把小鼠8-细胞以后胚胎划分为:8-细胞晚期[细胞数目为（11.9±0.65）个];同一时间收集的桑椹胚和早期囊胚细胞数目相似;不同时间收集的囊胚细胞有明显差异。（3）细胞计数分析表明:8-细胞聚合胚发育形成聚合囊胚时,其细胞数目未发生调整。Counting embryo cell number is a wildly used methods to exam embryo viablity. In this paper, We use the mouse embryo in vivo developed and aggregated embryo to investigate the cell number counting method. The result as follows: 1)The mounting method combind with hypo-tonic treatment was found to be superior for counting cell number of embryo to other methods; 2)The cell number analysis in vivo developingmouse embryos showed that: the cell number of the late 8 - cell mouse embryo is 11. 9 ?0. 65; the cell number was similar in morula and early blastocyst obtained in the same batch;there was significant difference in cell number between the blastocysts collected at different time although their morphology was similar;3)The cell number analyisi in aggregated mouse late 8 - cell embryo showed that in cell number no regulation had not occurred during the blastocyst period.

关 键 词：胚胎 细胞计数 小鼠 低渗压片法 

分 类 号：Q132[生物学—普通生物学] Q2－33