清毒饮和养正片诱导L_(7212)小鼠白血病细胞凋亡及其对Bcl-2、P^(53)基因表达的影响  

Qingdu Yin and Yangzheng Pian Induce Apoptosis of Leukemia Cell in L7212 Mice and Their Influence on Expression of Bcl - 2、p53 Gene

作　　者：杨洪涌[1] 丘和明[1] 陈志雄[1] 胡莉文[1] 李振波[1] 潘习龙[2] 

机构地区：[1]广州中医药大学第一附属医院,广东广州510405 [2]广州中医药大学,广东广州510405

出　　处：《新中医》2002年第6期69-71,共3页New Chinese Medicine

基　　金：国家中医药管理局青年课题(97Y030)

摘　　要：目的:探讨清毒饮和养正片抗白血病的作用机理。方法;采用L7212白血病小鼠模型,用原位末端标记法（TUNEL）观察清毒饮、养正片和化疗对其白血病细胞凋亡的影响。结果:清毒饮、养正片、化疗各组都可见较多白血病细胞凋亡。其中清毒饮组优于养正片组,合用化疗则更明显,凋亡指数均大于模型对照组（P<0.01）,证明清毒饮、养正片能诱导L7212白血病细胞凋亡,且以清毒饮较明显,并可提高化疗的促凋亡作用。用免疫组化法检测模型小鼠骨髓有核细胞中Bcl-2、P53基因的表达,其Bcl-2表达明显增高,予清毒饮、养正片处理后,Bcl-2有所下降,以清毒饮组较养正片组明显,合用化疗后下降更明显（P<0.05）;P53表达在L7212白血病细胞稍减低,经清毒饮、养正片、化疗处理后,表达阳性率及其强度均有所升高,但统计学处理差异无显著性意义（P>0.05）。结论:清毒饮、养正片确能下调Bcl-2表达,而对P53作用不够明显。ve: To explore the anti - leukemia mechanism of Qingdu Yin(QY) and Yangzheng Pian (YP). Methods: L7212 mice were adopted as the leukemia model. TUNEL was applied to observe the influences of QY, YP and chemotherapy (CT) on apoptosis of leukemia cells. Results: Apoptosis of leukemia cells were always ob-served in QY group, YP group and CT group, more superior in QY group than in YP group, especially more obvious in those combined with CT. The apoptotic indexes of above groups were larger than that of model group ( P < 0. 01). It was shown that QY and YP can induce apoptosis of L7212 leukemia cell and enhance the apoptotic action of CT. Immunohistochemistry showed that the Bcl - 2 gene expression of karyocytes in bone marrow of mice model was markedly increased, and decreased by QY and YP, especially combined with CT( P < 0. 05). The P53 gene expression of L7212 leukemia cell was slightly re-duced, and its positive rate and intensity was increased by QY, YP and CT, but the difference was not statistically significant ( P > 0. 05). Conclusion: QY and YP can lower the expression of Bcl - 2 but not act on P53.

关 键 词：基因表达 消毒饮 养正片 细胞凋亡 基因表达调控 白血病 疾病模型 Bcl-2基因 P53基因 药理学 

分 类 号：R273.37[医药卫生—中西医结合] R285[医药卫生—中医肿瘤科]